Structural study of the N-glycans of intercellular adhesion molecule-5 (telencephalin)

Tomohiro Ohgomori, Osamu Funatsu, Syu ichi Nakaya, Akinori Morita, Masahiko Ikekita

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Intercellular adhesion molecule-5 (ICAM-5, telencephalin) is a dendritically polarized membrane glycoprotein expressed in tissues distinct from those expressing other ICAMs. Here, we determined the N-glycan structure of ICAM-5 purified from adult rat brain and compared it with that of other ICAMs. N-glycans were released by N-glycosidase F digestion and labeled with p-amino benzoic octylester (ABOE). ABOE-labeled glycans were analyzed by high performance liquid chromatography (HPLC) and mass spectrometry. The N-glycans obtained from rat brain ICAM-5 consisted of approximately 85% neutral, 10.2% sialylated-only, 2.8% sulfated-only, and 1.2% sialylated and sulfated glycans. Compared with the N-glycan structures of human ICAM-1 expressed in CHO cells, HEK cells, or mouse myeloma cells and ICAM-3 isolated from human T-cells, rat brain ICAM-5 had less highly branched glycans, sialylated glycans, and N-acetyllactosamine structures. In contrast, high-mannose-type N-glycans and Lewis X were more commonly found in rat brain ICAM-5 than in human ICAM-1 expressed in CHO cells, HEK cells, or mouse myeloma cells and ICAM-3 isolated from human T-cells. In addition, sulfated glycans contained GlcNAc 6-O-sulfate on the non-reducing terminal side. Our data will be important for the elucidation of the roles of the N-glycans expressed in neural cells, including those present on ICAM-5.

Original languageEnglish
Pages (from-to)1611-1623
Number of pages13
JournalBiochimica et Biophysica Acta - General Subjects
Volume1790
Issue number12
DOIs
Publication statusPublished - Dec 1 2009
Externally publishedYes

Fingerprint

Cell Adhesion Molecules
Polysaccharides
Rats
Brain
T-cells
CHO Cells
Intercellular Adhesion Molecule-1
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
T-Lymphocytes
Membrane Glycoproteins
High performance liquid chromatography
Mannose
Mass spectrometry
Digestion
Mass Spectrometry
High Pressure Liquid Chromatography
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Structural study of the N-glycans of intercellular adhesion molecule-5 (telencephalin). / Ohgomori, Tomohiro; Funatsu, Osamu; Nakaya, Syu ichi; Morita, Akinori; Ikekita, Masahiko.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1790, No. 12, 01.12.2009, p. 1611-1623.

Research output: Contribution to journalArticle

Ohgomori, Tomohiro ; Funatsu, Osamu ; Nakaya, Syu ichi ; Morita, Akinori ; Ikekita, Masahiko. / Structural study of the N-glycans of intercellular adhesion molecule-5 (telencephalin). In: Biochimica et Biophysica Acta - General Subjects. 2009 ; Vol. 1790, No. 12. pp. 1611-1623.
@article{159c66cb00874355be0140700b78180e,
title = "Structural study of the N-glycans of intercellular adhesion molecule-5 (telencephalin)",
abstract = "Intercellular adhesion molecule-5 (ICAM-5, telencephalin) is a dendritically polarized membrane glycoprotein expressed in tissues distinct from those expressing other ICAMs. Here, we determined the N-glycan structure of ICAM-5 purified from adult rat brain and compared it with that of other ICAMs. N-glycans were released by N-glycosidase F digestion and labeled with p-amino benzoic octylester (ABOE). ABOE-labeled glycans were analyzed by high performance liquid chromatography (HPLC) and mass spectrometry. The N-glycans obtained from rat brain ICAM-5 consisted of approximately 85{\%} neutral, 10.2{\%} sialylated-only, 2.8{\%} sulfated-only, and 1.2{\%} sialylated and sulfated glycans. Compared with the N-glycan structures of human ICAM-1 expressed in CHO cells, HEK cells, or mouse myeloma cells and ICAM-3 isolated from human T-cells, rat brain ICAM-5 had less highly branched glycans, sialylated glycans, and N-acetyllactosamine structures. In contrast, high-mannose-type N-glycans and Lewis X were more commonly found in rat brain ICAM-5 than in human ICAM-1 expressed in CHO cells, HEK cells, or mouse myeloma cells and ICAM-3 isolated from human T-cells. In addition, sulfated glycans contained GlcNAc 6-O-sulfate on the non-reducing terminal side. Our data will be important for the elucidation of the roles of the N-glycans expressed in neural cells, including those present on ICAM-5.",
author = "Tomohiro Ohgomori and Osamu Funatsu and Nakaya, {Syu ichi} and Akinori Morita and Masahiko Ikekita",
year = "2009",
month = "12",
day = "1",
doi = "10.1016/j.bbagen.2009.08.012",
language = "English",
volume = "1790",
pages = "1611--1623",
journal = "Biochimica et Biophysica Acta - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "12",

}

TY - JOUR

T1 - Structural study of the N-glycans of intercellular adhesion molecule-5 (telencephalin)

AU - Ohgomori, Tomohiro

AU - Funatsu, Osamu

AU - Nakaya, Syu ichi

AU - Morita, Akinori

AU - Ikekita, Masahiko

PY - 2009/12/1

Y1 - 2009/12/1

N2 - Intercellular adhesion molecule-5 (ICAM-5, telencephalin) is a dendritically polarized membrane glycoprotein expressed in tissues distinct from those expressing other ICAMs. Here, we determined the N-glycan structure of ICAM-5 purified from adult rat brain and compared it with that of other ICAMs. N-glycans were released by N-glycosidase F digestion and labeled with p-amino benzoic octylester (ABOE). ABOE-labeled glycans were analyzed by high performance liquid chromatography (HPLC) and mass spectrometry. The N-glycans obtained from rat brain ICAM-5 consisted of approximately 85% neutral, 10.2% sialylated-only, 2.8% sulfated-only, and 1.2% sialylated and sulfated glycans. Compared with the N-glycan structures of human ICAM-1 expressed in CHO cells, HEK cells, or mouse myeloma cells and ICAM-3 isolated from human T-cells, rat brain ICAM-5 had less highly branched glycans, sialylated glycans, and N-acetyllactosamine structures. In contrast, high-mannose-type N-glycans and Lewis X were more commonly found in rat brain ICAM-5 than in human ICAM-1 expressed in CHO cells, HEK cells, or mouse myeloma cells and ICAM-3 isolated from human T-cells. In addition, sulfated glycans contained GlcNAc 6-O-sulfate on the non-reducing terminal side. Our data will be important for the elucidation of the roles of the N-glycans expressed in neural cells, including those present on ICAM-5.

AB - Intercellular adhesion molecule-5 (ICAM-5, telencephalin) is a dendritically polarized membrane glycoprotein expressed in tissues distinct from those expressing other ICAMs. Here, we determined the N-glycan structure of ICAM-5 purified from adult rat brain and compared it with that of other ICAMs. N-glycans were released by N-glycosidase F digestion and labeled with p-amino benzoic octylester (ABOE). ABOE-labeled glycans were analyzed by high performance liquid chromatography (HPLC) and mass spectrometry. The N-glycans obtained from rat brain ICAM-5 consisted of approximately 85% neutral, 10.2% sialylated-only, 2.8% sulfated-only, and 1.2% sialylated and sulfated glycans. Compared with the N-glycan structures of human ICAM-1 expressed in CHO cells, HEK cells, or mouse myeloma cells and ICAM-3 isolated from human T-cells, rat brain ICAM-5 had less highly branched glycans, sialylated glycans, and N-acetyllactosamine structures. In contrast, high-mannose-type N-glycans and Lewis X were more commonly found in rat brain ICAM-5 than in human ICAM-1 expressed in CHO cells, HEK cells, or mouse myeloma cells and ICAM-3 isolated from human T-cells. In addition, sulfated glycans contained GlcNAc 6-O-sulfate on the non-reducing terminal side. Our data will be important for the elucidation of the roles of the N-glycans expressed in neural cells, including those present on ICAM-5.

UR - http://www.scopus.com/inward/record.url?scp=71549148073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71549148073&partnerID=8YFLogxK

U2 - 10.1016/j.bbagen.2009.08.012

DO - 10.1016/j.bbagen.2009.08.012

M3 - Article

C2 - 19733219

AN - SCOPUS:71549148073

VL - 1790

SP - 1611

EP - 1623

JO - Biochimica et Biophysica Acta - General Subjects

JF - Biochimica et Biophysica Acta - General Subjects

SN - 0304-4165

IS - 12

ER -