Structure-activity relationship and inhibition pattern of reishi-derived (Ganoderma lingzhi) triterpenoids against angiotensin-converting enzyme

Tran Hai-Bang, Kuniyoshi Shimizu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Abstract Many triterpenoids have shown ability to inhibit hydrolyzing activity of angiotensin-converting enzyme; however, there has been no report about the structure-activity relationship and inhibition patterns of these compounds. In this study, 32 lanostane-type triterpenoids derived from Ganoderma lingzhi were assayed to determine the structural features involved in the observed inhibition effects. In silico and in vitro experiments were also used to determine the kinetics of the reaction. Fifteen compounds showed measurable in vitro IC50 values ranging from 0.194 to 0.941 mM. It was shown that carboxyl groups play an important role in inhibiting the enzyme; further, a hydroxyl group or carbonyl group at either C7 or C15 increases the inhibition rate, and a double bond at C24,25 decreases the activity. Based on the docking data we speculated that triterpenoids could not fit into the active site of the enzyme; therefore, the inhibition mode could not be competitive. Dixon plotting showed that the inhibition patterns should be uncompetitive and non-competitive instead.

Original languageEnglish
Article number940
Pages (from-to)243-247
Number of pages5
JournalPhytochemistry Letters
Volume12
DOIs
Publication statusPublished - Jun 16 2015

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Agronomy and Crop Science
  • Plant Science

Cite this