Structure and activity of JAC1 j-domain implicate the involvement of the cochaperone activity with Hsc70 in chloroplast photorelocation movement

Noriyuki Suetsugu, Akira Takano, Daisuke Kohda, Masamitsu Wada

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Chloroplast photorelocation movement towards weak light and away from strong light is essential for plants to adapt to the fluctuation of ambient light conditions. In the previous study, we showed that blue light receptor phototropins mediated blue light-induced chloroplast movement in Arabidopsis by regulating short actin filaments localized at the chloroplast periphery (cp-actin filaments) rather than actin cables in the cytoplasm. However, the signaling pathway for the chloroplast photorelocation movement is still unclear. We also identified JAC1 (J-domain protein required for chloroplast accumulation response 1) as an essential component for the accumulation response and dark positioning in Arabidopsis. We recently determined the crystal structure of the J-domain of JAC1. The JAC1 J-domain has a positively charged surface, which forms a putative interface with the Hsc70 chaperone by analogy to that of bovine auxilin. Furthermore, the mutation of the highly conserved HPD motif in the JAC1 J-domain impaired the in vivo activity of JAC1. These data suggest that JAC1 cochaperone activity with HSC70 is essential for chloroplast photorelocation movement.

    Original languageEnglish
    JournalPlant Signaling and Behavior
    Volume5
    Issue number12
    DOIs
    Publication statusPublished - Dec 1 2010

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    chloroplasts
    blue light
    microfilaments
    Arabidopsis
    crystal structure
    actin
    cytoplasm
    mutation
    receptors
    cattle
    proteins

    All Science Journal Classification (ASJC) codes

    • Plant Science

    Cite this

    Structure and activity of JAC1 j-domain implicate the involvement of the cochaperone activity with Hsc70 in chloroplast photorelocation movement. / Suetsugu, Noriyuki; Takano, Akira; Kohda, Daisuke; Wada, Masamitsu.

    In: Plant Signaling and Behavior, Vol. 5, No. 12, 01.12.2010.

    Research output: Contribution to journalArticle

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    abstract = "Chloroplast photorelocation movement towards weak light and away from strong light is essential for plants to adapt to the fluctuation of ambient light conditions. In the previous study, we showed that blue light receptor phototropins mediated blue light-induced chloroplast movement in Arabidopsis by regulating short actin filaments localized at the chloroplast periphery (cp-actin filaments) rather than actin cables in the cytoplasm. However, the signaling pathway for the chloroplast photorelocation movement is still unclear. We also identified JAC1 (J-domain protein required for chloroplast accumulation response 1) as an essential component for the accumulation response and dark positioning in Arabidopsis. We recently determined the crystal structure of the J-domain of JAC1. The JAC1 J-domain has a positively charged surface, which forms a putative interface with the Hsc70 chaperone by analogy to that of bovine auxilin. Furthermore, the mutation of the highly conserved HPD motif in the JAC1 J-domain impaired the in vivo activity of JAC1. These data suggest that JAC1 cochaperone activity with HSC70 is essential for chloroplast photorelocation movement.",
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    AU - Wada, Masamitsu

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