Abstract
The nucleotide sequences of the recessive dnaQ49 and the dominant mutD5 mutator were determined. The dnaQ49 mutator has a single base substitution in the dnaQ gene, thus causing one amino acid change,96Val (GTG)→ Gly (GGG), in the DnaQ protein (ε subunit of DNA polymerase III holoenzyme). The mutD5 mutator possesses two base substitutions in the same gene, resulting in two amino acid changes,73Leu (TTG)→Trp (TGG) and164Ala (GCA)→Val (GTA), which were designated the mutD52 and mutD51 mutations, respectively. Construction of chimaeric genes carrying one or two of these mutations revealed: (1) either mutD51 or mutD52 alone causes the dominant mutator phenotype when present in a multi-copy plasmid; (2)mutD51, but not mutD52, exerts the dominant mutator phenotype when present in a low-copy plasmid; (3) the dominant mutD51 mutator activity is suppressed by the dnaQ49 mutation when both mutations are present in the same gene. Based on these findings, we devised a model for the action of these mutators.
| Original language | English |
|---|---|
| Pages (from-to) | 9-13 |
| Number of pages | 5 |
| Journal | MGG Molecular & General Genetics |
| Volume | 205 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Oct 1 1986 |
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All Science Journal Classification (ASJC) codes
- Genetics
Cite this
Structure and function of dnaQ and mutD mutators of Escherichia coli. / Takano, Kyoko; Nakabeppu, Yusaku; Maki, Hisaji; Horiuchi, Takashi; Sekiguchi, Mutsuo.
In: MGG Molecular & General Genetics, Vol. 205, No. 1, 01.10.1986, p. 9-13.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Structure and function of dnaQ and mutD mutators of Escherichia coli
AU - Takano, Kyoko
AU - Nakabeppu, Yusaku
AU - Maki, Hisaji
AU - Horiuchi, Takashi
AU - Sekiguchi, Mutsuo
PY - 1986/10/1
Y1 - 1986/10/1
N2 - The nucleotide sequences of the recessive dnaQ49 and the dominant mutD5 mutator were determined. The dnaQ49 mutator has a single base substitution in the dnaQ gene, thus causing one amino acid change,96Val (GTG)→ Gly (GGG), in the DnaQ protein (ε subunit of DNA polymerase III holoenzyme). The mutD5 mutator possesses two base substitutions in the same gene, resulting in two amino acid changes,73Leu (TTG)→Trp (TGG) and164Ala (GCA)→Val (GTA), which were designated the mutD52 and mutD51 mutations, respectively. Construction of chimaeric genes carrying one or two of these mutations revealed: (1) either mutD51 or mutD52 alone causes the dominant mutator phenotype when present in a multi-copy plasmid; (2)mutD51, but not mutD52, exerts the dominant mutator phenotype when present in a low-copy plasmid; (3) the dominant mutD51 mutator activity is suppressed by the dnaQ49 mutation when both mutations are present in the same gene. Based on these findings, we devised a model for the action of these mutators.
AB - The nucleotide sequences of the recessive dnaQ49 and the dominant mutD5 mutator were determined. The dnaQ49 mutator has a single base substitution in the dnaQ gene, thus causing one amino acid change,96Val (GTG)→ Gly (GGG), in the DnaQ protein (ε subunit of DNA polymerase III holoenzyme). The mutD5 mutator possesses two base substitutions in the same gene, resulting in two amino acid changes,73Leu (TTG)→Trp (TGG) and164Ala (GCA)→Val (GTA), which were designated the mutD52 and mutD51 mutations, respectively. Construction of chimaeric genes carrying one or two of these mutations revealed: (1) either mutD51 or mutD52 alone causes the dominant mutator phenotype when present in a multi-copy plasmid; (2)mutD51, but not mutD52, exerts the dominant mutator phenotype when present in a low-copy plasmid; (3) the dominant mutD51 mutator activity is suppressed by the dnaQ49 mutation when both mutations are present in the same gene. Based on these findings, we devised a model for the action of these mutators.
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U2 - 10.1007/BF02428026
DO - 10.1007/BF02428026
M3 - Article
VL - 205
SP - 9
EP - 13
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
SN - 1617-4615
IS - 1
ER -