Structure and interaction in lipid bilayers analyzed using bicelles

Research output: Contribution to journalReview article

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Abstract

Although many non-peptidic drugs target biological membrane and membrane proteins, it is still difficult to determine the membrane-bound conformation of the drugs. To solve those problems, we have utilized bicelles as a membrane model, since the bicelles, which have planar lipid bilayer portions, are thought to be a more appropriate membrane model than micelles. Small-sized bicelles allow for liquid NMR measurements due to isotropic fast tumbling in solution. We have applied small bicelles to erythromycin A, salinomycin, and amphidinol 3, and determined their membrane-bound structures as well as their positions and orientations in the membranes using coupling constants, NOEs, and paramagnetic relaxation methods. Recently, we found that sphingomyelin, a major lipid constituent of lipid rafts, also forms bicelles, and established its conformation in the bicelles. These studies show the general utility of small bicelles for detailed conformation and orientation analysis of membrane-associated drugs and lipid molecules. We are now developing a bicelle-based crystallization method for membrane proteins, which will facilitate the cocrystalization of membrane proteins and hydrophobic drugs.

Original languageEnglish
Pages (from-to)596-603
Number of pages8
JournalYuki Gosei Kagaku Kyokaishi/Journal of Synthetic Organic Chemistry
Volume72
Issue number5
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

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Lipid bilayers
Membranes
Conformations
Membrane Proteins
Lipids
Pharmaceutical Preparations
Biological membranes
Barreling
Sphingomyelins
Micelles
Erythromycin
Crystallization
Crystal orientation
Nuclear magnetic resonance
Molecules
Liquids

All Science Journal Classification (ASJC) codes

  • Organic Chemistry

Cite this

Structure and interaction in lipid bilayers analyzed using bicelles. / Matsumori, Nobuaki.

In: Yuki Gosei Kagaku Kyokaishi/Journal of Synthetic Organic Chemistry, Vol. 72, No. 5, 01.01.2014, p. 596-603.

Research output: Contribution to journalReview article

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