Structure and ligand recognition of the PB1 domain: A novel protein module binding to the PC motif

Hiroaki Terasawa, Yukiko Noda, Takashi Ito, Hideki Hatanaka, Saori Ichikawa, Kenji Ogura, Hideki Sumimoto, Fuyuhiko Inagaki

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

PB1 domains are novel protein modules capable of binding to target proteins that contain PC motifs. We report here the NMR structure and ligand-binding site of the PB1 domain of the cell polarity establishment protein, Bem1p. In addition, we identify the topology of the PC motif-containing region of Cdc24p by NMR, another cell polarity establishment protein that interacts with Bem1p. The PC motif-containing region is a structural domain offering a scaffold to the PC motif. The chemical shift perturbation experiment and the mutagenesis study show that the PC motif is a major structural element that binds to the PB1 domain. A structural database search reveals close similarity between the Bem1p PB1 domain and the c-Raf1 Ras-binding domain. However, these domains are functionally distinct from each other.

Original languageEnglish
Pages (from-to)3947-3956
Number of pages10
JournalEMBO Journal
Volume20
Issue number15
DOIs
Publication statusPublished - Aug 1 2001

Fingerprint

Protein Binding
Cell Polarity
Ligands
Proteins
Nuclear magnetic resonance
Mutagenesis
Chemical shift
Binding Sites
Scaffolds
Databases
Topology
Experiments

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Terasawa, H., Noda, Y., Ito, T., Hatanaka, H., Ichikawa, S., Ogura, K., ... Inagaki, F. (2001). Structure and ligand recognition of the PB1 domain: A novel protein module binding to the PC motif. EMBO Journal, 20(15), 3947-3956. https://doi.org/10.1093/emboj/20.15.3947

Structure and ligand recognition of the PB1 domain : A novel protein module binding to the PC motif. / Terasawa, Hiroaki; Noda, Yukiko; Ito, Takashi; Hatanaka, Hideki; Ichikawa, Saori; Ogura, Kenji; Sumimoto, Hideki; Inagaki, Fuyuhiko.

In: EMBO Journal, Vol. 20, No. 15, 01.08.2001, p. 3947-3956.

Research output: Contribution to journalArticle

Terasawa, H, Noda, Y, Ito, T, Hatanaka, H, Ichikawa, S, Ogura, K, Sumimoto, H & Inagaki, F 2001, 'Structure and ligand recognition of the PB1 domain: A novel protein module binding to the PC motif', EMBO Journal, vol. 20, no. 15, pp. 3947-3956. https://doi.org/10.1093/emboj/20.15.3947
Terasawa, Hiroaki ; Noda, Yukiko ; Ito, Takashi ; Hatanaka, Hideki ; Ichikawa, Saori ; Ogura, Kenji ; Sumimoto, Hideki ; Inagaki, Fuyuhiko. / Structure and ligand recognition of the PB1 domain : A novel protein module binding to the PC motif. In: EMBO Journal. 2001 ; Vol. 20, No. 15. pp. 3947-3956.
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