Structure and promoter analysis of the cytochrome P-450 aromatase gene of the teleost fish, medaka (Oryzias latipes)

Minoru Tanaka, Sachiko Fukada, Michiya Matsuyama, Yoshitaka Nagahama

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

We isolated the structural gene encoding cytochrome P-450 aromatase (P-450arom), for the first time from a nonmammalian vertebrate, the medaka (a teleost fish, Oryzias latipes), using the rainbow trout P-450arom cDNA as a probe. The structure of the entire P-450arom gene, the nucleotide sequence of its 5' flanking region, and the transcriptional initiation sites were determined. The medaka P-450arom gene consists of nine exons, but spans only 2.6 kb, being much smaller than the human P-450arom gene (at least 70 kb), as a result of extremely small introns (medaka, 73-213 bp vs. human, 1.3-10 kbp). The splicing junctions are located at exactly the same positions as those found in the human P-450arom. The deduced amino acid sequence is 51-52% identical to those of mammals and chicken, and 75% identical to the rainbow trout amino acid sequence. Genomic Southern blots revealed the presence of a single medaka gene. Promoter analyses indicated two major transcription initiation sites 60 and 61 bp upstream from a putative initiation codon. The promoter region of medaka P-450arom gene also contains potential Ad4BP sites and estrogen responsive element (ERE) half-sites. These results suggest that the basic structural organization of P-450arom genes and the regulatory mechanisms of expression are well conserved throughout the vertebrates

Original languageEnglish
Pages (from-to)719-725
Number of pages7
JournalJournal of biochemistry
Volume117
Issue number4
DOIs
Publication statusPublished - Jan 1 1995
Externally publishedYes

Fingerprint

Oryzias
Aromatase
Fish
Cytochrome P-450 Enzyme System
Fishes
Genes
Oncorhynchus mykiss
Vertebrates
Amino Acid Sequence
Amino Acids
Gene encoding
Mammals
Initiator Codon
5' Flanking Region
Transcription Initiation Site
Regulator Genes
Southern Blotting
Genetic Promoter Regions
Introns
Chickens

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Structure and promoter analysis of the cytochrome P-450 aromatase gene of the teleost fish, medaka (Oryzias latipes). / Tanaka, Minoru; Fukada, Sachiko; Matsuyama, Michiya; Nagahama, Yoshitaka.

In: Journal of biochemistry, Vol. 117, No. 4, 01.01.1995, p. 719-725.

Research output: Contribution to journalArticle

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