Structure and reaction mechanism of heme oxygenase-1

Hiroshi Sakamoto, Masakazu Sugishima, Yoshiaki Omata, Yoshimitsu Kakuta, Keiichi Fukuyama, Graham Palmer, Masato Noguchi

Research output: Contribution to journalArticle

Abstract

Heme oxygenase (HO) catalyzes the oxidative cleavage of heme to yield biliverdin, CO and iron. It is established that heme degrades via two intermediates: α-hydroxyheme and verdoheme, however, the redox stoichiometry for the overall HO reaction remains unclear. We determined the crystal structure of the rat HO-1 in complex with heme and found that the proximal ligand of the heme iron is His-25. At the distal side, no side chains made direct contact with the heme iron but a hydroxide that can be stabilized by hydrogen bonds formed with backbones of Gly-139 and Gly-143. One of the hydrogen bonds would facilitate the electrophilic addition of the ferric hydroperoxide to the porphyrin ring, resulting in heme α-meso-hydroxylation. Furthermore, we prepared a ferric α-hydroxyheme–HO-1 complex and titrated the complex with O2 under strictly anaerobic conditions. The results obtained indicate that the α-hydroxyheme bound to the enzyme can be converted to a verdoheme by an approximately equimolar amount of O2, without any requirement for exogenous electrons. The redox state of the verdoheme formed proved to be ferrous when CO or potassium ferricyanide was added to the resultant verdoheme–HO-1 complex.

Original languageEnglish
Pages (from-to)177-183
Number of pages7
JournalInternational Congress Series
Volume1233
Issue numberC
DOIs
Publication statusPublished - Nov 1 2002

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Heme Oxygenase-1
Heme
Heme Oxygenase (Decyclizing)
Iron
Carbon Monoxide
Oxidation-Reduction
Hydrogen
Biliverdine
Porphyrins
Hydroxylation
Hydrogen Peroxide
Electrons
Ligands
Enzymes
verdoheme

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Sakamoto, H., Sugishima, M., Omata, Y., Kakuta, Y., Fukuyama, K., Palmer, G., & Noguchi, M. (2002). Structure and reaction mechanism of heme oxygenase-1. International Congress Series, 1233(C), 177-183. https://doi.org/10.1016/S0531-5131(02)00273-X

Structure and reaction mechanism of heme oxygenase-1. / Sakamoto, Hiroshi; Sugishima, Masakazu; Omata, Yoshiaki; Kakuta, Yoshimitsu; Fukuyama, Keiichi; Palmer, Graham; Noguchi, Masato.

In: International Congress Series, Vol. 1233, No. C, 01.11.2002, p. 177-183.

Research output: Contribution to journalArticle

Sakamoto, H, Sugishima, M, Omata, Y, Kakuta, Y, Fukuyama, K, Palmer, G & Noguchi, M 2002, 'Structure and reaction mechanism of heme oxygenase-1', International Congress Series, vol. 1233, no. C, pp. 177-183. https://doi.org/10.1016/S0531-5131(02)00273-X
Sakamoto H, Sugishima M, Omata Y, Kakuta Y, Fukuyama K, Palmer G et al. Structure and reaction mechanism of heme oxygenase-1. International Congress Series. 2002 Nov 1;1233(C):177-183. https://doi.org/10.1016/S0531-5131(02)00273-X
Sakamoto, Hiroshi ; Sugishima, Masakazu ; Omata, Yoshiaki ; Kakuta, Yoshimitsu ; Fukuyama, Keiichi ; Palmer, Graham ; Noguchi, Masato. / Structure and reaction mechanism of heme oxygenase-1. In: International Congress Series. 2002 ; Vol. 1233, No. C. pp. 177-183.
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