Structure determination of the major component of the starfish ganglioside molecular species LG-2 by tandem mass spectrometry

Ryuichi Higuchi, Shuji Matsumoto, Ryuichi Isobe, Tomofumi Miyamoto

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The biologically active ganglioside molecular species, LG-2, which has been obtained from the starfish Astropecten latespinosus, was completely methylated followed by separation using reversed phase HPLC to give a methylated major component, LG-2M-5, in the pure state. The ceramide moiety of LG-2M-5 was indicated by the positive ion fast atom bombardment tandem mass spectrometry (FABMS/MS) of LG-2M-5. This fact was verified from the measurements of the positive ion FABMS/MS of the permethylates prepared from four known starfish cerebrosides.

Original languageEnglish
Pages (from-to)8961-8968
Number of pages8
JournalTetrahedron
Volume51
Issue number33
DOIs
Publication statusPublished - Aug 14 1995

Fingerprint

Starfish
Gangliosides
Tandem Mass Spectrometry
Mass spectrometry
Positive ions
Ions
Cerebrosides
Fast Atom Bombardment Mass Spectrometry
Ceramides
High Pressure Liquid Chromatography
Atoms

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

Cite this

Structure determination of the major component of the starfish ganglioside molecular species LG-2 by tandem mass spectrometry. / Higuchi, Ryuichi; Matsumoto, Shuji; Isobe, Ryuichi; Miyamoto, Tomofumi.

In: Tetrahedron, Vol. 51, No. 33, 14.08.1995, p. 8961-8968.

Research output: Contribution to journalArticle

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