Structure, development, and maintenance of the nerve net of the body column in Hydra

M. Sakaguchi, A. Mizusina, Yoshitaka Kobayakawa

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The anatomy and developmental dynamics of the nerve net in the body column of Hydra viridissima were examined immunocytochemically with a monoclonal antibody (CC04) that recognizes an antigen in nerve cells and with an antiserum against vasopressin. CC04+ neuron cell bodies, their neurites, and vasopressin-like-immunoreactive (VLI+) neurites could be clearly visualized on whole-mount preparations. All neurites of the CC04+ neurons in the body column were VLI+. However, only half of the VLI+ neurites in the body column were CC04+. Immunocytochemical analysis of macerated preparations showed that half of the neurons in the gastric region of the body column were CC04+. These results suggest that most of the neurons in the gastric region are VLI+. The density of the VLI+ neurites was uniform along the entire length of the body column. The CC04+ neuron density in the gastric region remained constant at all stages of asexual development and during foot regeneration. After pulse-labeling with 5-bromo-2'-deoxyuridine (BrdU), CC04+ neurons with labeled nuclei appeared in the body column. We conclude that neuron density in the gastric region is maintained at a constant value by insertion of new neurons in parallel with continuous epithelial cell division.

Original languageEnglish
Pages (from-to)41-54
Number of pages14
JournalJournal of Comparative Neurology
Volume373
Issue number1
DOIs
Publication statusPublished - Sep 9 1996

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Nerve Net
Hydra
Maintenance
Neurons
Neurites
Stomach
Vasopressins
Body Regions
Bromodeoxyuridine
Cell Division
Foot
Immune Sera
Regeneration
Anatomy

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Structure, development, and maintenance of the nerve net of the body column in Hydra. / Sakaguchi, M.; Mizusina, A.; Kobayakawa, Yoshitaka.

In: Journal of Comparative Neurology, Vol. 373, No. 1, 09.09.1996, p. 41-54.

Research output: Contribution to journalArticle

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