Structure elucidation of uniformly 13C-labeled bacterial celluloses from different Gluconacetobacter xylinus strains

Stephanie Hesse-Ertelt, Thomas Heinze, Eiji Togawa, Tetsuo Kondo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The morphological and supramolecular structures of native cellulose pellicles from two strains of Gluconacetobacter xylinus (ATCC 53582, ATCC 23769) were investigated. Samples had been statically cultivated in Hestrin-Schramm medium containing fully 13C-labeled β-d-glucose-U-13C6 as the sole source of carbon. The results are compared with structure data of bacterial celluloses with a natural 13C abundance of 1.1%. Non-enriched and 13C-labeled cellulose pellicles formed crystalline structures as revealed by cross-polarized/magic-angle spinning (CP/MAS) 13C{1H}-NMR and near infrared (NIR) FT-Raman spectroscopic measurements as well as wide-angle X-ray diffraction (WAXD) investigations. Atomic force microscopy (AFM) was applied for analyzing fiber morphologies and surface properties. For the first time, details about the manipulation of fiber widths and pellicle formation were shown for different bacterial strains of G. xylinus depending on the use of β-d-glucose-U-13C6 for the biosynthesis.

Original languageEnglish
Pages (from-to)139-151
Number of pages13
JournalCellulose
Volume17
Issue number1
DOIs
Publication statusPublished - Feb 1 2010

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Cellulose
Glucose
Magic angle spinning
Fibers
Biosynthesis
Surface properties
Atomic force microscopy
Carbon
Nuclear magnetic resonance
Crystalline materials
Infrared radiation
X ray diffraction

All Science Journal Classification (ASJC) codes

  • Polymers and Plastics

Cite this

Structure elucidation of uniformly 13C-labeled bacterial celluloses from different Gluconacetobacter xylinus strains. / Hesse-Ertelt, Stephanie; Heinze, Thomas; Togawa, Eiji; Kondo, Tetsuo.

In: Cellulose, Vol. 17, No. 1, 01.02.2010, p. 139-151.

Research output: Contribution to journalArticle

Hesse-Ertelt, Stephanie ; Heinze, Thomas ; Togawa, Eiji ; Kondo, Tetsuo. / Structure elucidation of uniformly 13C-labeled bacterial celluloses from different Gluconacetobacter xylinus strains. In: Cellulose. 2010 ; Vol. 17, No. 1. pp. 139-151.
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