Structure of β-1,3-xylooligosaccharides generated from Caulerpa racemosa var. laete-virens β-1,3-xylan by the action of β-1,3-xylanase

Masashi Kiyohara, Yoichiro Hama, Kuniko Yamaguchi, Makoto Ito

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Recently we reported the molecular cloning and characterization of a novel β-1,3-xylanase from the marine bacterium Vibrio sp. AX-4 [Kiyohara et al. (2005) Biochem. J. 388, 949-957]. We report here the structural analysis of oligosaccharides generated from β-1,3-xylan of a siphonous green alga, Caulerpa racemosa var. laete-virens, by the action of β-1,3-xylanase. The enzyme degraded the polysaccharide producing oligosaccharides with different Rfs on TLC (EX2-EX5). Sugar component, linkage, and MALDI-TOF-MS analyses revealed that EX2 and EX3 were Xyl-1,3-Xyl and Xyl-1,3-Xyl-1,3-Xyl, respectively. On the other hand, EX4 was a mixture of Glc-1,3-Xyl-1,3-Xyl, Xyl-1,4-Xyl-1,3-Xyl and Xyl-1,3-Xyl-1,4-Xyl, while EX5 was a mixture of tetra-saccharides containing 3-substitued GIc in addition to the same components of EX4. Branching was not likely present in EXOs prepared from the polysaccharide by the enzyme. These results strongly suggest that the C. racemosa β-1,3-xylan is a linear heteropolysaccharide containing 1,3-Glc and 1,4-Xyl both of which are thought to be located within a β-1,3-Xyl chain and linked via covalent bonds. This report indicates the usefulness of the enzyme for the structural analysis of β-1,3-xylan.

Original languageEnglish
Pages (from-to)369-373
Number of pages5
JournalJournal of Biochemistry
Volume140
Issue number3
DOIs
Publication statusPublished - Sep 1 2006

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Caulerpa
Oligosaccharides
Structural analysis
Polysaccharides
Enzymes
Chlorophyta
Covalent bonds
Vibrio
Cloning
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Molecular Cloning
Algae
Sugars
Bacteria
1,3-xylan
xylooligosaccharide

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

Structure of β-1,3-xylooligosaccharides generated from Caulerpa racemosa var. laete-virens β-1,3-xylan by the action of β-1,3-xylanase. / Kiyohara, Masashi; Hama, Yoichiro; Yamaguchi, Kuniko; Ito, Makoto.

In: Journal of Biochemistry, Vol. 140, No. 3, 01.09.2006, p. 369-373.

Research output: Contribution to journalArticle

Kiyohara, Masashi ; Hama, Yoichiro ; Yamaguchi, Kuniko ; Ito, Makoto. / Structure of β-1,3-xylooligosaccharides generated from Caulerpa racemosa var. laete-virens β-1,3-xylan by the action of β-1,3-xylanase. In: Journal of Biochemistry. 2006 ; Vol. 140, No. 3. pp. 369-373.
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