Structure of an antibody in complex with its mucin domain linear epitope that is protective against ebola virus

Daniel Olal, Ana I. Kuehne, Shridhar Bale, Peter Halfmann, Takao Hashiguchi, Marnie L. Fusco, Jeffrey E. Lee, Liam B. King, Yoshihiro Kawaoka, John M. Dye, Erica Ollmann Saphire

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33 Citations (Scopus)

Abstract

Antibody 14G7 is protective against lethal Ebola virus challenge and recognizes a distinct linear epitope in the prominent mucinlike domain of the Ebola virus glycoprotein GP. The structure of 14G7 in complex with its linear peptide epitope has now been determined to 2.8 A°. The structure shows that this GP sequence forms a tandemβ-hairpin structure that binds deeply into a cleft in the antibody-combining site. A key threonine at the apex of one turn is critical for antibody interaction and is conserved among all Ebola viruses. This work provides further insight into the mechanism of protection by antibodies that target the protruding, highly accessible mucin-like domain of Ebola virus and the structural framework for understanding and characterizing candidate immunotherapeutics.

Original languageEnglish
Pages (from-to)2809-2816
Number of pages8
JournalJournal of virology
Volume86
Issue number5
DOIs
Publication statusPublished - Mar 2012

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Olal, D., Kuehne, A. I., Bale, S., Halfmann, P., Hashiguchi, T., Fusco, M. L., Lee, J. E., King, L. B., Kawaoka, Y., Dye, J. M., & Saphire, E. O. (2012). Structure of an antibody in complex with its mucin domain linear epitope that is protective against ebola virus. Journal of virology, 86(5), 2809-2816. https://doi.org/10.1128/JVI.05549-11