Structure of the human CCG1 gene: relationship between the exons/introns and functional domain/modules of the protein

Nakashima Torahiko, Sekiguchi Takeshi, Sunamoto Hidetoshi, Yura Kei, Tomoda Shirou, Go Mitiko, Kere Juha, Schlessinger David, Nishimoto Takeharu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The human CCG1 gene, encoding CCGl/TAFII250/p250, was isolated by complementing tsBN462, a mutant BHK21 cell line that shows cell-cycle arrest at high temperature. Using the cDNA as a probe, the locations of exon-intron junctions were determined in the genomic DNA. Thirty-eight exons ranging from 68 to 219 bp in size were found. All the exonintron junctions followed the GT-AG rule. Using a newly developed method, we performed a module analysis of the CCG1 protein. The functional domain previously predicted in CCG1 was further confirmed to be encoded in a single predicted module that is the minimal functional unit in the protein. The boundaries of the predicted modules show a close correlation to the intron/exon junction of CCG1. The entire gene, at least 110kb long, has been recovered in a YAC, which provides a route to the further study of module function.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalGene
Volume141
Issue number2
DOIs
Publication statusPublished - Apr 20 1994

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Introns
Exons
Genes
Cell Cycle Checkpoints
Proteins
Complementary DNA
Cell Line
Temperature
DNA
Protein Domains

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Structure of the human CCG1 gene : relationship between the exons/introns and functional domain/modules of the protein. / Torahiko, Nakashima; Takeshi, Sekiguchi; Hidetoshi, Sunamoto; Kei, Yura; Shirou, Tomoda; Mitiko, Go; Juha, Kere; David, Schlessinger; Takeharu, Nishimoto.

In: Gene, Vol. 141, No. 2, 20.04.1994, p. 193-200.

Research output: Contribution to journalArticle

Torahiko, N, Takeshi, S, Hidetoshi, S, Kei, Y, Shirou, T, Mitiko, G, Juha, K, David, S & Takeharu, N 1994, 'Structure of the human CCG1 gene: relationship between the exons/introns and functional domain/modules of the protein', Gene, vol. 141, no. 2, pp. 193-200. https://doi.org/10.1016/0378-1119(94)90570-3
Torahiko, Nakashima ; Takeshi, Sekiguchi ; Hidetoshi, Sunamoto ; Kei, Yura ; Shirou, Tomoda ; Mitiko, Go ; Juha, Kere ; David, Schlessinger ; Takeharu, Nishimoto. / Structure of the human CCG1 gene : relationship between the exons/introns and functional domain/modules of the protein. In: Gene. 1994 ; Vol. 141, No. 2. pp. 193-200.
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AU - Kei, Yura

AU - Shirou, Tomoda

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AU - Juha, Kere

AU - David, Schlessinger

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