Structure of the human prealbumin gene

Teruhisa Tsuzuki, S. Mita, S. Maeda, S. Araki, K. Shimada

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Using cloned human prealbumin cDNA as a probe, Southern blot hybridization of human genomic DNA revealed that the prealbumin gene consists of an unique, single-copy DNA. The nucleotide sequences of the entire human prealbumin gene, including both 581 base pairs of the 5'- and 95 base pairs of the 3'-flanking sequences, were determined. The gene spans about 7.0 kilobase pairs and consists of four exons and three introns. As in most eukaryotic genes, the consensus TATA and CAAT sequences were found 30 and 101 nucleotides, respectively, upstream from the putative cap site, and a polyadenylation signal sequence AATAAA is found in the 3'-untranslated region. Unexpectedly, two independent open reading frames provided with respective regulatory sequences were found within the gene: one in the first intron and the other in the third intron.

Original languageEnglish
Pages (from-to)12224-12227
Number of pages4
JournalJournal of Biological Chemistry
Volume260
Issue number22
Publication statusPublished - Dec 1 1985

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Tsuzuki, T., Mita, S., Maeda, S., Araki, S., & Shimada, K. (1985). Structure of the human prealbumin gene. Journal of Biological Chemistry, 260(22), 12224-12227.