Structure of the Membrane Domain of Human Erythrocyte Anion Exchanger 1 Revealed by Electron Crystallography

Tomohiro Yamaguchi, Yohei Ikeda, Yoshito Abe, Hiroyuki Kuma, Dongchon Kang, Naotaka Hamasaki, Teruhisa Hirai

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The membrane domain of human erythrocyte anion exchanger 1 (AE1) works as a Cl-/HCO3- antiporter. This exchange is a key step for CO2/O2 circulation in the blood. In spite of their importance, structural information about AE1 and the AE (anion exchanger) family are still very limited. We used electron microscopy to solve the three-dimensional structure of the AE1 membrane domain, fixed in an outward-open conformation by cross-linking, at 7.5-Å resolution. A dimer of AE1 membrane domains packed in two-dimensional array showed a projection map similar to that of the prokaryotic homolog of the ClC chloride channel, a Cl-/H+ antiporter. In a three-dimensional map, there are V-shaped densities near the center of the dimer and slightly narrower V-shaped clusters at a greater distance from the center of the dimer. These appear to be inserted into the membrane from opposite sides. The structural motifs, two homologous pairs of helices in internal repeats of the ClC transporter (helices B + C and J + K), are well fitted to those AE1 densities after simple domain movement.

Original languageEnglish
Pages (from-to)179-189
Number of pages11
JournalJournal of Molecular Biology
Volume397
Issue number1
DOIs
Publication statusPublished - Mar 19 2010

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Erythrocyte Anion Exchange Protein 1
Crystallography
Electrons
Membranes
Antiporters
Chloride Channels
Blood Circulation
Anions
Electron Microscopy

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Structure of the Membrane Domain of Human Erythrocyte Anion Exchanger 1 Revealed by Electron Crystallography. / Yamaguchi, Tomohiro; Ikeda, Yohei; Abe, Yoshito; Kuma, Hiroyuki; Kang, Dongchon; Hamasaki, Naotaka; Hirai, Teruhisa.

In: Journal of Molecular Biology, Vol. 397, No. 1, 19.03.2010, p. 179-189.

Research output: Contribution to journalArticle

Yamaguchi, Tomohiro ; Ikeda, Yohei ; Abe, Yoshito ; Kuma, Hiroyuki ; Kang, Dongchon ; Hamasaki, Naotaka ; Hirai, Teruhisa. / Structure of the Membrane Domain of Human Erythrocyte Anion Exchanger 1 Revealed by Electron Crystallography. In: Journal of Molecular Biology. 2010 ; Vol. 397, No. 1. pp. 179-189.
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