Structure of the N-terminal SH3 domain of GRB2 complexed with a peptide from the guanine nucleotide releasing factor Sos

Hiroaki Terasawa, Daisuke Kohda, Hideki Hatanaka, Shigeo Tsuchiya, Kenji Ogura, Koji Nagata, Shunsuke Ishii, Valsan Mandiyan, Axel Ullrich, Joseph Schlessinger, Fuyuhiko Inagaki

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Src-homology 3 (SH3) domains mediate signal transduction by binding to proline- rich motifs in target proteins. We have determined the high-resolution NMR structure of the complex between the amino-terminal SH3 domain of GRB2 and a ten amino acid peptide derived from the guanine nucleotide releasing factor Sos. The NMR data show that the peptide adopts the conformation of a left-handed polyproline type II helix and interacts with three major sites on the SH3 domain. The orientation of the bound peptide is opposite to that of proline-rich peptides bound to the SH3 domains of Abl, Fyn and p85.

Original languageEnglish
Pages (from-to)891-997
Number of pages107
JournalNature Structural Biology
Volume1
Issue number12
DOIs
Publication statusPublished - Dec 1994

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Guanine Nucleotide Exchange Factors
src Homology Domains
Peptides
Proline
Nuclear magnetic resonance
Signal transduction
Conformations
Signal Transduction
Amino Acids
Proteins

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biochemistry
  • Genetics

Cite this

Structure of the N-terminal SH3 domain of GRB2 complexed with a peptide from the guanine nucleotide releasing factor Sos. / Terasawa, Hiroaki; Kohda, Daisuke; Hatanaka, Hideki; Tsuchiya, Shigeo; Ogura, Kenji; Nagata, Koji; Ishii, Shunsuke; Mandiyan, Valsan; Ullrich, Axel; Schlessinger, Joseph; Inagaki, Fuyuhiko.

In: Nature Structural Biology, Vol. 1, No. 12, 12.1994, p. 891-997.

Research output: Contribution to journalArticle

Terasawa, H, Kohda, D, Hatanaka, H, Tsuchiya, S, Ogura, K, Nagata, K, Ishii, S, Mandiyan, V, Ullrich, A, Schlessinger, J & Inagaki, F 1994, 'Structure of the N-terminal SH3 domain of GRB2 complexed with a peptide from the guanine nucleotide releasing factor Sos', Nature Structural Biology, vol. 1, no. 12, pp. 891-997. https://doi.org/10.1038/nsb1294-891
Terasawa, Hiroaki ; Kohda, Daisuke ; Hatanaka, Hideki ; Tsuchiya, Shigeo ; Ogura, Kenji ; Nagata, Koji ; Ishii, Shunsuke ; Mandiyan, Valsan ; Ullrich, Axel ; Schlessinger, Joseph ; Inagaki, Fuyuhiko. / Structure of the N-terminal SH3 domain of GRB2 complexed with a peptide from the guanine nucleotide releasing factor Sos. In: Nature Structural Biology. 1994 ; Vol. 1, No. 12. pp. 891-997.
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