Structure of the [NiFe]-hydrogenase maturation protein HypF from Thermococcus kodakarensis KOD1

Taiga Tominaga, Satoshi Watanabe, Rie Matsumi, Haruyuki Atomi, Tadayuki Imanaka, Kunio Miki

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

HypF is involved in the biosynthesis of the CN ligand of the NiFe(CN)2CO centre of [NiFe]-hydrogenases. Here, the full-length structure of HypF from Thermococcus kodakarenesis is reported at 4.5 Å resolution. The N-terminal acylphosphatase-like (ACP) domain interacts with the zinc-finger domain with some flexibility in its relative position. Molecular-surface analysis shows that a deep pocket formed between the ACP and zinc-finger domains is highly conserved and has positive potential. These results suggest that the positively charged pocket identified is involved in the hydrolysis of carbamoyl phosphate and the formation of a carbamoyl intermediate.

Original languageEnglish
Pages (from-to)1153-1157
Number of pages5
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume68
Issue number10
DOIs
Publication statusPublished - Oct 1 2012

Fingerprint

Thermococcus
Zinc Fingers
Zinc
Carbamyl Phosphate
proteins
Biosynthesis
Surface analysis
zinc
Hydrolysis
Proteins
biosynthesis
Ligands
hydrolysis
phosphates
flexibility
ligands
acylphosphatase
nickel-iron hydrogenase

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

Structure of the [NiFe]-hydrogenase maturation protein HypF from Thermococcus kodakarensis KOD1. / Tominaga, Taiga; Watanabe, Satoshi; Matsumi, Rie; Atomi, Haruyuki; Imanaka, Tadayuki; Miki, Kunio.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 68, No. 10, 01.10.2012, p. 1153-1157.

Research output: Contribution to journalArticle

Tominaga, Taiga ; Watanabe, Satoshi ; Matsumi, Rie ; Atomi, Haruyuki ; Imanaka, Tadayuki ; Miki, Kunio. / Structure of the [NiFe]-hydrogenase maturation protein HypF from Thermococcus kodakarensis KOD1. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2012 ; Vol. 68, No. 10. pp. 1153-1157.
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