Structure-specific DNA nucleases: Structural basis for 3D-scissors

Tatsuya Nishino, Yoshizumi Ishino, Kosuke Morikawa

Research output: Contribution to journalReview article

22 Citations (Scopus)

Abstract

Structure-specific DNA nucleases play important roles in various DNA transactions such as DNA replication, repair and recombination. These enzymes recognize loops and branched DNA structures. Recent structural studies have provided detailed insights into the functions of these enzymes. Structures of Holliday junction resolvase revealed that nucleases are broadly diverged in the way in which they fold, however, are required to form homodimers with large basic patches of protein surfaces, which are complementary to DNA tertiary structures. Many nucleases maintain structure-specific recognition modes, which involve particular domain arrangements through conformal changes of flexible loops or have a separate DNA binding domain. Nucleases, such as FEN-1 and archaeal XPF, are bound to proliferating cell nuclear antigen through a common motif, and thereby actualize their inherent activities.

Original languageEnglish
Pages (from-to)60-67
Number of pages8
JournalCurrent Opinion in Structural Biology
Volume16
Issue number1
DOIs
Publication statusPublished - Feb 1 2006

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Deoxyribonucleases
Holliday Junction Resolvases
DNA
Flap Endonucleases
Proliferating Cell Nuclear Antigen
Enzymes
DNA Replication
DNA Repair
Genetic Recombination
Membrane Proteins
Complementary DNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Structural Biology

Cite this

Structure-specific DNA nucleases : Structural basis for 3D-scissors. / Nishino, Tatsuya; Ishino, Yoshizumi; Morikawa, Kosuke.

In: Current Opinion in Structural Biology, Vol. 16, No. 1, 01.02.2006, p. 60-67.

Research output: Contribution to journalReview article

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