Structure-specific DNA nucleases: Structural basis for 3D-scissors

Tatsuya Nishino, Yoshizumi Ishino, Kosuke Morikawa

Research output: Contribution to journalReview articlepeer-review

24 Citations (Scopus)


Structure-specific DNA nucleases play important roles in various DNA transactions such as DNA replication, repair and recombination. These enzymes recognize loops and branched DNA structures. Recent structural studies have provided detailed insights into the functions of these enzymes. Structures of Holliday junction resolvase revealed that nucleases are broadly diverged in the way in which they fold, however, are required to form homodimers with large basic patches of protein surfaces, which are complementary to DNA tertiary structures. Many nucleases maintain structure-specific recognition modes, which involve particular domain arrangements through conformal changes of flexible loops or have a separate DNA binding domain. Nucleases, such as FEN-1 and archaeal XPF, are bound to proliferating cell nuclear antigen through a common motif, and thereby actualize their inherent activities.

Original languageEnglish
Pages (from-to)60-67
Number of pages8
JournalCurrent Opinion in Structural Biology
Issue number1
Publication statusPublished - Feb 2006

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology


Dive into the research topics of 'Structure-specific DNA nucleases: Structural basis for 3D-scissors'. Together they form a unique fingerprint.

Cite this