Structures of cDNAs encoding the muscle-type and non-muscle-type isozymes of lamprey fructose bisphosphate aldolases and the evolution of aldolase genes

Rong Zhang, Hitomi Yatsuki, Takahiro Kusakabe, Naoyuki Iwabe, Takashi Miyata, Toshio Imai, Mitsutaka Yoshida, Katsuji Hori

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Nearly full-length cDNA clones for muscle-type and non-muscle-type aldolase mRNAs were cloned from λgt10 cDNA libraries constructed from skeletal muscle and liver mRNAs of lamprey (Entosphenus japonicus). The cDNA-M8 has 2,240 bp carrying an open reading frame of 1,089 bp which encodes 362 amino acids without the amino terminal methionine, while the cDNA-L3 is 1,761 bp in length and has an open reading frame of 1,092 bp, which encodes 363 amino acids without the methionine. We designated the cDNA clones M8 and L3 as the muscle-type and non-muscle-type aldolase cDNAs, respectively. The entire amino acid sequences deduced from cDNA-M8 and -L3 show a high degree of identity to one another (76%) and also to vertebrate aldolases A (74-76%), B (68-70%), and C (71-76%) and Drosophila melanogaster aldolases α, β, and γ (66-67%). Northern blot analyses using the 3'-noncoding sequences of cDNA-M8 and -L3 as hybridization probes indicated that the muscle-type mRNA is expressed mainly in the skeletal muscle, heart muscle, brain, and some other tissues, but probably not in liver, while the non-muscle-type mRNA is expressed mainly in the liver and also in brain and other tissues, except for the heart muscle. Phylogenetic analyses showed that both muscle-type and non-muscle-type aldolases of lamprey resemble one another and might share a common ancestor with vertebrate aldolases A and C, but they are not direct ancestors of vertebrate aldolases.

Original languageEnglish
Pages (from-to)545-553
Number of pages9
JournalJournal of biochemistry
Volume117
Issue number3
DOIs
Publication statusPublished - Jan 1 1995

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Lampreys
Fructose-Bisphosphate Aldolase
Isoenzymes
Muscle
Complementary DNA
Genes
Aldehyde-Lyases
Muscles
Vertebrates
Liver
Messenger RNA
Methionine
Open Reading Frames
Amino Acids
Myocardium
Skeletal Muscle
Clone Cells
Brain
Tissue
Drosophila melanogaster

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Structures of cDNAs encoding the muscle-type and non-muscle-type isozymes of lamprey fructose bisphosphate aldolases and the evolution of aldolase genes. / Zhang, Rong; Yatsuki, Hitomi; Kusakabe, Takahiro; Iwabe, Naoyuki; Miyata, Takashi; Imai, Toshio; Yoshida, Mitsutaka; Hori, Katsuji.

In: Journal of biochemistry, Vol. 117, No. 3, 01.01.1995, p. 545-553.

Research output: Contribution to journalArticle

Zhang, Rong ; Yatsuki, Hitomi ; Kusakabe, Takahiro ; Iwabe, Naoyuki ; Miyata, Takashi ; Imai, Toshio ; Yoshida, Mitsutaka ; Hori, Katsuji. / Structures of cDNAs encoding the muscle-type and non-muscle-type isozymes of lamprey fructose bisphosphate aldolases and the evolution of aldolase genes. In: Journal of biochemistry. 1995 ; Vol. 117, No. 3. pp. 545-553.
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abstract = "Nearly full-length cDNA clones for muscle-type and non-muscle-type aldolase mRNAs were cloned from λgt10 cDNA libraries constructed from skeletal muscle and liver mRNAs of lamprey (Entosphenus japonicus). The cDNA-M8 has 2,240 bp carrying an open reading frame of 1,089 bp which encodes 362 amino acids without the amino terminal methionine, while the cDNA-L3 is 1,761 bp in length and has an open reading frame of 1,092 bp, which encodes 363 amino acids without the methionine. We designated the cDNA clones M8 and L3 as the muscle-type and non-muscle-type aldolase cDNAs, respectively. The entire amino acid sequences deduced from cDNA-M8 and -L3 show a high degree of identity to one another (76{\%}) and also to vertebrate aldolases A (74-76{\%}), B (68-70{\%}), and C (71-76{\%}) and Drosophila melanogaster aldolases α, β, and γ (66-67{\%}). Northern blot analyses using the 3'-noncoding sequences of cDNA-M8 and -L3 as hybridization probes indicated that the muscle-type mRNA is expressed mainly in the skeletal muscle, heart muscle, brain, and some other tissues, but probably not in liver, while the non-muscle-type mRNA is expressed mainly in the liver and also in brain and other tissues, except for the heart muscle. Phylogenetic analyses showed that both muscle-type and non-muscle-type aldolases of lamprey resemble one another and might share a common ancestor with vertebrate aldolases A and C, but they are not direct ancestors of vertebrate aldolases.",
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