TY - JOUR
T1 - Studies on DNA-related enzymes to elucidate molecular mechanisms underlying genetic information processing and their application in genetic engineering
AU - Ishino, Yoshizumi
N1 - Funding Information:
This work was supported by JSPS KAKENHI as well as JST BIRD, JST CREST, HFSP Research Grant, and others. I learned organic chemistry and biochemistry of nucleic acids under Drs. Morio Ikehara and Eiko Otsuka of the Faculty of Pharmaceutical Sciences and learned molecular genetics under Drs. Atsuo Nakata and Hideo Shinagawa at Research Institute for Microbial Diseases, Osaka University. I studied about RNA enzymes under Dr. Dieter Söll at Yale University. At the research institute in Takara Shuzo under director Akira Obayashi and later Ikunoshin Kato, I led a research group on genetic engineering enzymes and PCR enzymes. I started the basic molecular biology of Archaea at Biomolecular Engineering Research Institute (BERI) under Director Yoshiro Shimura and Kosuke Morikawa. Based on these experiences, I have been working on the basic and applied research of hyperthermophilic archaea since I was appointed a full-time professor at the Faculty of Agriculture, Kyushu University. I would like to thank all those who provided research environment, guidance, and support as well as the lab members of Osaka University, Takara Shuzo, BERI, and Kyushu University and the collaborators who worked with me. I also thank my colleagues belonging to the Archaea research field worldwide, particularly Drs. Patrick Forterre (Institute Pasteur), Francine Perler (New England Biolab), Isaac Cann (Univ of Illinois) and Haruyuki Atomi (Kyoto Univ), who always provided critical discussion and encouragement. Finally, I would like to thank my colleagues at the Faculty of Agriculture, Kyushu University, particularly Dr. Toshihisa Oshima for his special encouragement.
Publisher Copyright:
© 2020 Japan Society for Bioscience, Biotechnology, and Agrochemistry.
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Recombinant DNA technology, in which artificially “cut and pasted” DNA in vitro is introduced into living cells, contributed extensively to the rapid development of molecular biology over the past 5 decades since the latter half of the 20th century. Although the original technology required special experiences and skills, the development of polymerase chain reaction (PCR) has greatly eased in vitro genetic manipulation for various experimental methods. The current development of a simple genome-editing technique using CRISPR-Cas9 gave great impetus to molecular biology. Genome editing is a major technique for elucidating the functions of many unknown genes. Genetic manipulation technologies rely on enzymes that act on DNA. It involves artificially synthesizing, cleaving, and ligating DNA strands by making good use of DNA-related enzymes present in organisms to maintain their life activities. In this review, I focus on key enzymes involved in the development of genetic manipulation technologies.
AB - Recombinant DNA technology, in which artificially “cut and pasted” DNA in vitro is introduced into living cells, contributed extensively to the rapid development of molecular biology over the past 5 decades since the latter half of the 20th century. Although the original technology required special experiences and skills, the development of polymerase chain reaction (PCR) has greatly eased in vitro genetic manipulation for various experimental methods. The current development of a simple genome-editing technique using CRISPR-Cas9 gave great impetus to molecular biology. Genome editing is a major technique for elucidating the functions of many unknown genes. Genetic manipulation technologies rely on enzymes that act on DNA. It involves artificially synthesizing, cleaving, and ligating DNA strands by making good use of DNA-related enzymes present in organisms to maintain their life activities. In this review, I focus on key enzymes involved in the development of genetic manipulation technologies.
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U2 - 10.1080/09168451.2020.1778441
DO - 10.1080/09168451.2020.1778441
M3 - Review article
C2 - 32567488
AN - SCOPUS:85087352674
SN - 0916-8451
VL - 84
SP - 1749
EP - 1766
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 9
ER -