Studies on molecular structure of biocompatible polymers by vibrational spectroscopy [24] ATR-IR studied of protein adsorption onto biocompatible polymer

Shoji Deguchi, Shigeaki Morita, Masaru Tanaka, Yukihiro Ozaki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Time-resolved IR spectra of protein adsorption process onto a polymer surface from buffer solution have been investigated by using an original in-situ attenuated total reflection infrared (ATR-IR) cell (Figure 1). The signal from adsorbed protein was selectively observed, i.e., that from natural protein in solution was not detected by the method. Figure 2 shows that the amide I bands (1700-1600cm-1) and the OD stretching band (2150-2200cm-1) regions of time-resolved spectra. Increase of amide I band and decrease of OD stretching band with time were observed (Figure 3). This result indicates that water molecules existing on the surface were desorbed by protein adsorption onto polymer surface. Protein adsorption from the aspect of water molecules will be discussed in detail.

Original languageEnglish
Title of host publication55th SPSJ Symposium on Macromolecules
Pages4379-4380
Number of pages2
Volume55
Edition2
Publication statusPublished - 2006
Externally publishedYes
Event55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan
Duration: Sep 20 2006Sep 22 2006

Other

Other55th Society of Polymer Science Japan Symposium on Macromolecules
CountryJapan
CityToyama
Period9/20/069/22/06

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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    Deguchi, S., Morita, S., Tanaka, M., & Ozaki, Y. (2006). Studies on molecular structure of biocompatible polymers by vibrational spectroscopy [24] ATR-IR studied of protein adsorption onto biocompatible polymer. In 55th SPSJ Symposium on Macromolecules (2 ed., Vol. 55, pp. 4379-4380)