Studies on the αL-iduronidase activity of β-glucuronidase preparations from bovine liver, rat liver, and rat preputial gland

Hiroko Kosaka, Mamoru Isemura, Teruo Ono, Yukio Nishimura, Keitaro Kato

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A commercial preparation of bovine liver β-glucuronidase contained two distinct enzyme species, both of which catalyze the hydrolysis of 4-methylumbelliferyl α-L-iduronide. The species with a molecular weight of about 290,000 was devoid of phenyl α-L-iduronidase activity and exhibited 4-methylumbelliferyl β-D-glucuronidase activity. The species with a molecular weight of about 78,000 was active towards phenyl α-L-iduronide but lacked the latter activity. Studies of the kinetics of inhibition and heat inactivation suggested that the hydrolysis of 4-methylumbelliferyl α-L-iduronide is due to the β-glucuronidase in the case of the 290,000-dalton species. The highly purified β-glucuronidase preparations derived from rat preputial gland and liver lysosomes also exhibited 4-methylumbelliferyl α-L-iduronidase activity. These findings support the view that β-glucuronidase can hydrolyze certain α-L-iduronide bonds and raise the possibility that β-glucuronidase may play a role in the catabolism of iduronic acid-containing glycosaminoglycans.

Original languageEnglish
Pages (from-to)69-75
Number of pages7
JournalJournal of biochemistry
Volume88
Issue number1
Publication statusPublished - Jul 1 1980

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Iduronidase
Glucuronidase
Liver
Rats
Hydrolysis
Iduronic Acid
Molecular Weight
Molecular weight
Lysosomes
Glycosaminoglycans
Hot Temperature
Kinetics
Enzymes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Studies on the αL-iduronidase activity of β-glucuronidase preparations from bovine liver, rat liver, and rat preputial gland. / Kosaka, Hiroko; Isemura, Mamoru; Ono, Teruo; Nishimura, Yukio; Kato, Keitaro.

In: Journal of biochemistry, Vol. 88, No. 1, 01.07.1980, p. 69-75.

Research output: Contribution to journalArticle

Kosaka, Hiroko ; Isemura, Mamoru ; Ono, Teruo ; Nishimura, Yukio ; Kato, Keitaro. / Studies on the αL-iduronidase activity of β-glucuronidase preparations from bovine liver, rat liver, and rat preputial gland. In: Journal of biochemistry. 1980 ; Vol. 88, No. 1. pp. 69-75.
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