Studies on the base excision repair (BER) complex in Pyrococcus furiosus

Shinichi Kiyonari, Saki Tahara, Maiko Uchimura, Tsuyoshi Shirai, Sonoko Ishino, Yoshizumi Ishino

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We have been studying the functions of PCNA (proliferating-cell nuclear antigen) for the assembly and reassembly of the replisome during replication fork progression. We have identified the functional interactions between PCNA and several proteins involved in DNA replication and repair from Pyrococcus furiosus. We recently reported that the activity of UDG (uracil-DNA glycosylase) in P. furiosus (PfuUDG) is stimulated by PCNA (PfuPCNA) in vitro, and identified an atypical PCNA-binding site, AKTLF, in the PfuUDG protein. To understand further the function of the complex in the BER (base excision repair) process, we investigated the AP (apurinic/apyrimidinic) endonuclease, which can process the BER pathway after uracil removal by UDG. Interestingly, one candidate ORF (open reading frame) for the AP endonuclease was found in the operon containing the gene encoding UDG in the P. furiosus genome. However, this ORF did not exhibit any activity. Instead, we identified the AP endonuclease activity from the other candidate gene products, and designated the protein as PfuAP. We discovered a physical interaction between PfuAP and PfuPCNA, suggesting the formation of a BER complex in one of the repair systems in P. furiosus.

Original languageEnglish
Pages (from-to)79-82
Number of pages4
JournalBiochemical Society Transactions
Volume37
Issue number1
DOIs
Publication statusPublished - Jun 26 2009

All Science Journal Classification (ASJC) codes

  • Biochemistry

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