Studies on the endothelin-1-induced contraction of rat granulation tissue pouch mediated by myofibroblasts

Takako Goto, Fumi Takahashi, Iwao Ohtsuki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A granulation tissue pouch, mostly composed of myofibroblasts, was prepared by injecting rats subcutaneously with croton oil. The contraction of the granulation tissue pouch caused by endothelin-1 (ET-1) and the effects of the ET receptor antagonists, BQ123 and BQ788 on it were thus examined. ET-1 produced contractions in a dose-dependent manner. Pretreatment with BQ123, an ET(A) receptor selective antagonist, shifted the dose-response curve to the right, whereas pretreatment with BQ788, an ET(B) receptor selective antagonist, showed little effect. IRL1620, an ET(B) receptor selective agonist, did not cause any contraction in the granulation tissue pouch. The existence of both ET(A) and ET(B) receptors in the granulation tissue pouch and in cultured myofibroblasts was demonstrated by RT-PCR. Intracellular Ca2+ mobilization in Fura-2/acetyl-methoxy ester loaded cultured myofibroblasts isolated from the granulation tissue was also examined. ET-1 produced a transient increase in [Ca2+](i) followed by a sustained elevation of [Ca2+](i), whereas IRL1620 caused only a transient peak. These results suggest that the ET-1 induced contraction of granulation tissue is mainly mediated through the mobilization of Ca2+ from the extracellular space caused by stimulation with ET(A) receptor. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)55-66
Number of pages12
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1405
Issue number1-3
DOIs
Publication statusPublished - Oct 21 1998

Fingerprint

Myofibroblasts
Granulation Tissue
Endothelin-1
Croton Oil
Fura-2
Extracellular Space
Esters
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Studies on the endothelin-1-induced contraction of rat granulation tissue pouch mediated by myofibroblasts. / Goto, Takako; Takahashi, Fumi; Ohtsuki, Iwao.

In: Biochimica et Biophysica Acta - Molecular Cell Research, Vol. 1405, No. 1-3, 21.10.1998, p. 55-66.

Research output: Contribution to journalArticle

@article{3cf7633deef94a8c819bc7b9f4dd3cd8,
title = "Studies on the endothelin-1-induced contraction of rat granulation tissue pouch mediated by myofibroblasts",
abstract = "A granulation tissue pouch, mostly composed of myofibroblasts, was prepared by injecting rats subcutaneously with croton oil. The contraction of the granulation tissue pouch caused by endothelin-1 (ET-1) and the effects of the ET receptor antagonists, BQ123 and BQ788 on it were thus examined. ET-1 produced contractions in a dose-dependent manner. Pretreatment with BQ123, an ET(A) receptor selective antagonist, shifted the dose-response curve to the right, whereas pretreatment with BQ788, an ET(B) receptor selective antagonist, showed little effect. IRL1620, an ET(B) receptor selective agonist, did not cause any contraction in the granulation tissue pouch. The existence of both ET(A) and ET(B) receptors in the granulation tissue pouch and in cultured myofibroblasts was demonstrated by RT-PCR. Intracellular Ca2+ mobilization in Fura-2/acetyl-methoxy ester loaded cultured myofibroblasts isolated from the granulation tissue was also examined. ET-1 produced a transient increase in [Ca2+](i) followed by a sustained elevation of [Ca2+](i), whereas IRL1620 caused only a transient peak. These results suggest that the ET-1 induced contraction of granulation tissue is mainly mediated through the mobilization of Ca2+ from the extracellular space caused by stimulation with ET(A) receptor. Copyright (C) 1998 Elsevier Science B.V.",
author = "Takako Goto and Fumi Takahashi and Iwao Ohtsuki",
year = "1998",
month = "10",
day = "21",
doi = "10.1016/S0167-4889(98)00105-0",
language = "English",
volume = "1405",
pages = "55--66",
journal = "Biochimica et Biophysica Acta - Molecular Cell Research",
issn = "0167-4889",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Studies on the endothelin-1-induced contraction of rat granulation tissue pouch mediated by myofibroblasts

AU - Goto, Takako

AU - Takahashi, Fumi

AU - Ohtsuki, Iwao

PY - 1998/10/21

Y1 - 1998/10/21

N2 - A granulation tissue pouch, mostly composed of myofibroblasts, was prepared by injecting rats subcutaneously with croton oil. The contraction of the granulation tissue pouch caused by endothelin-1 (ET-1) and the effects of the ET receptor antagonists, BQ123 and BQ788 on it were thus examined. ET-1 produced contractions in a dose-dependent manner. Pretreatment with BQ123, an ET(A) receptor selective antagonist, shifted the dose-response curve to the right, whereas pretreatment with BQ788, an ET(B) receptor selective antagonist, showed little effect. IRL1620, an ET(B) receptor selective agonist, did not cause any contraction in the granulation tissue pouch. The existence of both ET(A) and ET(B) receptors in the granulation tissue pouch and in cultured myofibroblasts was demonstrated by RT-PCR. Intracellular Ca2+ mobilization in Fura-2/acetyl-methoxy ester loaded cultured myofibroblasts isolated from the granulation tissue was also examined. ET-1 produced a transient increase in [Ca2+](i) followed by a sustained elevation of [Ca2+](i), whereas IRL1620 caused only a transient peak. These results suggest that the ET-1 induced contraction of granulation tissue is mainly mediated through the mobilization of Ca2+ from the extracellular space caused by stimulation with ET(A) receptor. Copyright (C) 1998 Elsevier Science B.V.

AB - A granulation tissue pouch, mostly composed of myofibroblasts, was prepared by injecting rats subcutaneously with croton oil. The contraction of the granulation tissue pouch caused by endothelin-1 (ET-1) and the effects of the ET receptor antagonists, BQ123 and BQ788 on it were thus examined. ET-1 produced contractions in a dose-dependent manner. Pretreatment with BQ123, an ET(A) receptor selective antagonist, shifted the dose-response curve to the right, whereas pretreatment with BQ788, an ET(B) receptor selective antagonist, showed little effect. IRL1620, an ET(B) receptor selective agonist, did not cause any contraction in the granulation tissue pouch. The existence of both ET(A) and ET(B) receptors in the granulation tissue pouch and in cultured myofibroblasts was demonstrated by RT-PCR. Intracellular Ca2+ mobilization in Fura-2/acetyl-methoxy ester loaded cultured myofibroblasts isolated from the granulation tissue was also examined. ET-1 produced a transient increase in [Ca2+](i) followed by a sustained elevation of [Ca2+](i), whereas IRL1620 caused only a transient peak. These results suggest that the ET-1 induced contraction of granulation tissue is mainly mediated through the mobilization of Ca2+ from the extracellular space caused by stimulation with ET(A) receptor. Copyright (C) 1998 Elsevier Science B.V.

UR - http://www.scopus.com/inward/record.url?scp=0031731808&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031731808&partnerID=8YFLogxK

U2 - 10.1016/S0167-4889(98)00105-0

DO - 10.1016/S0167-4889(98)00105-0

M3 - Article

VL - 1405

SP - 55

EP - 66

JO - Biochimica et Biophysica Acta - Molecular Cell Research

JF - Biochimica et Biophysica Acta - Molecular Cell Research

SN - 0167-4889

IS - 1-3

ER -