Studies on the formation mechanism and the structure of the anisotropic collagen gel prepared by dialysis-induced anisotropic gelation

Kazuya Furusawa, Shoichi Sato, Jyun Ichi Masumoto, Yohei Hanazaki, Yasuyuki Maki, Toshiaki Dobashi, Takao Yamamoto, Akimasa Fukui, Naoki Sasaki

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We have found that dialysis of 5 mg/mL collagen solution into the phosphate solution with a pH of 7.1 and an ionic strength of 256 mM at 25 °C results in a collagen gel with a birefringence and tubular pores aligned parallel to the growth direction of the gel. The time course of averaged diameter of tubular pores during the anisotropic gelation was expressed by a power law with an exponent of 1/3, suggesting that the formation of tubular pores is attributed to a spinodal decomposition-like phase separation. Small angle light scattering patterns and high resolution confocal laser scanning microscope images of the anisotropic collagen gel suggested that the collagen fibrils are aligned perpendicular to the growth direction of the gel. The positional dependence of the order parameter of the collagen fibrils showed that the anisotropic collagen gel has an orientation gradient.

Original languageEnglish
Pages (from-to)29-39
Number of pages11
JournalBiomacromolecules
Volume13
Issue number1
DOIs
Publication statusPublished - Jan 9 2012
Externally publishedYes

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Dialysis
Gelation
Collagen
Gels
Spinodal decomposition
Birefringence
Ionic strength
Phase separation
Light scattering
Phosphates
Microscopes
Scanning
Lasers

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Studies on the formation mechanism and the structure of the anisotropic collagen gel prepared by dialysis-induced anisotropic gelation. / Furusawa, Kazuya; Sato, Shoichi; Masumoto, Jyun Ichi; Hanazaki, Yohei; Maki, Yasuyuki; Dobashi, Toshiaki; Yamamoto, Takao; Fukui, Akimasa; Sasaki, Naoki.

In: Biomacromolecules, Vol. 13, No. 1, 09.01.2012, p. 29-39.

Research output: Contribution to journalArticle

Furusawa, K, Sato, S, Masumoto, JI, Hanazaki, Y, Maki, Y, Dobashi, T, Yamamoto, T, Fukui, A & Sasaki, N 2012, 'Studies on the formation mechanism and the structure of the anisotropic collagen gel prepared by dialysis-induced anisotropic gelation', Biomacromolecules, vol. 13, no. 1, pp. 29-39. https://doi.org/10.1021/bm200869p
Furusawa, Kazuya ; Sato, Shoichi ; Masumoto, Jyun Ichi ; Hanazaki, Yohei ; Maki, Yasuyuki ; Dobashi, Toshiaki ; Yamamoto, Takao ; Fukui, Akimasa ; Sasaki, Naoki. / Studies on the formation mechanism and the structure of the anisotropic collagen gel prepared by dialysis-induced anisotropic gelation. In: Biomacromolecules. 2012 ; Vol. 13, No. 1. pp. 29-39.
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AU - Maki, Yasuyuki

AU - Dobashi, Toshiaki

AU - Yamamoto, Takao

AU - Fukui, Akimasa

AU - Sasaki, Naoki

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