Studies on the mechanism for cryogel formation in vitro

Keiichi Miyamoto, Yoshiyuki Shimonishi, Keiichi Miyashita, Masayuki Tokita, Eiji Shakashita, Takashi Komai

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Abstract

We have been reported that the cryogel obtained from plasma with rheumatoid arthritis are mainly composed of three components; fibronectin(FN), fibrinogen(Fbg) and heparin(Hep). The concentration of cellular EDA(+)FN in cryogel was much higher compared with that in plasma. In order to investigate the mechanism of the cryogel-formation, we have attempted to reform cryogel in the solution composed of those proteins and polysaccharide by cooling in vitro. The hydrodynamic radius of the reformed gels were studied by dynamic light scattering method and the concentrations of the gels reformed in solution were obtained by the measurement of turbidity by use of the laser light. As a result, we found that the gel was reformed at the component ratio as 1(FN)/1(Hep)/15(Fbg) and 5-20 (EDA(+)/100(FN) in solution.

Original languageEnglish
Pages (from-to)465-471
Number of pages7
JournalJapanese Journal of Artificial Organs
Volume26
Issue number2
Publication statusPublished - Jan 1 1997

All Science Journal Classification (ASJC) codes

  • Biophysics

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    Miyamoto, K., Shimonishi, Y., Miyashita, K., Tokita, M., Shakashita, E., & Komai, T. (1997). Studies on the mechanism for cryogel formation in vitro. Japanese Journal of Artificial Organs, 26(2), 465-471.