We have been reported that the cryogel obtained from plasma with rheumatoid arthritis are mainly composed of three components; fibronectin(FN), fibrinogen(Fbg) and heparin(Hep). The concentration of cellular EDA(+)FN in cryogel was much higher compared with that in plasma. In order to investigate the mechanism of the cryogel-formation, we have attempted to reform cryogel in the solution composed of those proteins and polysaccharide by cooling in vitro. The hydrodynamic radius of the reformed gels were studied by dynamic light scattering method and the concentrations of the gels reformed in solution were obtained by the measurement of turbidity by use of the laser light. As a result, we found that the gel was reformed at the component ratio as 1(FN)/1(Hep)/15(Fbg) and 5-20 (EDA(+)/100(FN) in solution.
|Number of pages||7|
|Journal||Japanese Journal of Artificial Organs|
|Publication status||Published - Jan 1 1997|
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