Studies on the mechanism for cryogel formation in vitro

Keiichi Miyamoto, Yoshiyuki Shimonishi, Keiichi Miyashita, Masayuki Tokita, Eiji Shakashita, Takashi Komai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have been reported that the cryogel obtained from plasma with rheumatoid arthritis are mainly composed of three components; fibronectin(FN), fibrinogen(Fbg) and heparin(Hep). The concentration of cellular EDA(+)FN in cryogel was much higher compared with that in plasma. In order to investigate the mechanism of the cryogel-formation, we have attempted to reform cryogel in the solution composed of those proteins and polysaccharide by cooling in vitro. The hydrodynamic radius of the reformed gels were studied by dynamic light scattering method and the concentrations of the gels reformed in solution were obtained by the measurement of turbidity by use of the laser light. As a result, we found that the gel was reformed at the component ratio as 1(FN)/1(Hep)/15(Fbg) and 5-20 (EDA(+)/100(FN) in solution.

Original languageEnglish
Pages (from-to)465-471
Number of pages7
JournalJapanese Journal of Artificial Organs
Volume26
Issue number2
Publication statusPublished - Jan 1 1997

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Cryogels
Fibronectins
Gels
Fibrinogen
Heparin
Hydrodynamics
Polysaccharides
Rheumatoid Arthritis
Lasers
Light
In Vitro Techniques
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

Miyamoto, K., Shimonishi, Y., Miyashita, K., Tokita, M., Shakashita, E., & Komai, T. (1997). Studies on the mechanism for cryogel formation in vitro. Japanese Journal of Artificial Organs, 26(2), 465-471.

Studies on the mechanism for cryogel formation in vitro. / Miyamoto, Keiichi; Shimonishi, Yoshiyuki; Miyashita, Keiichi; Tokita, Masayuki; Shakashita, Eiji; Komai, Takashi.

In: Japanese Journal of Artificial Organs, Vol. 26, No. 2, 01.01.1997, p. 465-471.

Research output: Contribution to journalArticle

Miyamoto, K, Shimonishi, Y, Miyashita, K, Tokita, M, Shakashita, E & Komai, T 1997, 'Studies on the mechanism for cryogel formation in vitro', Japanese Journal of Artificial Organs, vol. 26, no. 2, pp. 465-471.
Miyamoto K, Shimonishi Y, Miyashita K, Tokita M, Shakashita E, Komai T. Studies on the mechanism for cryogel formation in vitro. Japanese Journal of Artificial Organs. 1997 Jan 1;26(2):465-471.
Miyamoto, Keiichi ; Shimonishi, Yoshiyuki ; Miyashita, Keiichi ; Tokita, Masayuki ; Shakashita, Eiji ; Komai, Takashi. / Studies on the mechanism for cryogel formation in vitro. In: Japanese Journal of Artificial Organs. 1997 ; Vol. 26, No. 2. pp. 465-471.
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