TY - JOUR
T1 - Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1
T2 - I. Isolation and some properties of the glycoproteins
AU - Jikibara, Takayuki
AU - Takegawa, Kaoru
AU - Iwahara, Shojiro
PY - 1992/1/1
Y1 - 1992/1/1
N2 - An acidic heteropolysaccharide preparation derived from the mycelium of Fusarium sp. M7-1 was fractionated into two fractions, precipitable and nonprecipitable, by treatment with cetyltrimethylammonium bromide (Cetavlon). These two fractions were further purified to apparent homogeneity on ultracentrifugation by treatment with charcoal and gel filtration chromatographies. Two glycoproteins, precipitable GP I and nonprecipitable GP II, were obtained. The molecular weighs of GP I and GP II were estimated to be about 8.8×104 and 3.7×104 respectively, on gel filtration chromatography. Both GP I and GP II contained a high proportion of serine and threonine. Treatment of GP I and GP II with alkaline solution resulted in an increase in absorbance at 240 nm. Alkaline borohydride treatment markedly decreased the number of Beryl and threonyl residues and resulted in an increase in alanine and the formation of 2-aminobutyric acid. It also resulted in release of low and high molecular weight carbohydrate chains. From these results, we conclude that both GP I and GP II are glycoproteins with carbohydrate chains attached to the protein moiety through O-glycosidic linkages to the hydroxyl group of serine and/or threonine.
AB - An acidic heteropolysaccharide preparation derived from the mycelium of Fusarium sp. M7-1 was fractionated into two fractions, precipitable and nonprecipitable, by treatment with cetyltrimethylammonium bromide (Cetavlon). These two fractions were further purified to apparent homogeneity on ultracentrifugation by treatment with charcoal and gel filtration chromatographies. Two glycoproteins, precipitable GP I and nonprecipitable GP II, were obtained. The molecular weighs of GP I and GP II were estimated to be about 8.8×104 and 3.7×104 respectively, on gel filtration chromatography. Both GP I and GP II contained a high proportion of serine and threonine. Treatment of GP I and GP II with alkaline solution resulted in an increase in absorbance at 240 nm. Alkaline borohydride treatment markedly decreased the number of Beryl and threonyl residues and resulted in an increase in alanine and the formation of 2-aminobutyric acid. It also resulted in release of low and high molecular weight carbohydrate chains. From these results, we conclude that both GP I and GP II are glycoproteins with carbohydrate chains attached to the protein moiety through O-glycosidic linkages to the hydroxyl group of serine and/or threonine.
UR - http://www.scopus.com/inward/record.url?scp=0026500323&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026500323&partnerID=8YFLogxK
U2 - 10.1093/oxfordjournals.jbchem.a123741
DO - 10.1093/oxfordjournals.jbchem.a123741
M3 - Article
C2 - 1569046
AN - SCOPUS:0026500323
VL - 111
SP - 225
EP - 229
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 2
ER -