Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: III. The primary structures of the acidic polysaccharides of the glycoproteins

Takayuki Jikibara, Kaoru Takegawa, Shojiro Iwahara

Research output: Contribution to journalArticle

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Abstract

Two O-glycosidically linked acidic polysaccharides, AP I and AP II, were released, respectively, from glycoproteins GP I and GP II of Fusarium sp. M7-1 by alkaline borohydride treatment and purified by gel filtration chromatography. They were found to be apparently homogeneous on gel filtration chromatography and analytical ultracentrifugation. Their molecular weights were estimated to be 8.2×104 and 3.1×104 respectively. The various oligosaccharide fragments obtained from AP I and AP II by acetolysis and partial acid hydrolysis were purified by gel filtration chromatography and HPLC. Their primary structures were resolved mainly by NMR spectrometry in combination with methylation mass spectrometry. Analyses of the acetolysis and partial acid hydrolysis products and the 1H-NMR spectrum of AP I and AP II showed that they are analogues. Thus, we propose that the main parts of the acidic polysaccharides have the following structures.*X, unidentified oligosaccharide chains. The numbers on the left and the numbers in parentheses outside the brackets indicate the approximate number of side chains of AP I and AP II, respectively, the saccharide sequences of which are not specified.

Original languageEnglish
Pages (from-to)236-243
Number of pages8
JournalJournal of biochemistry
Volume111
Issue number2
DOIs
Publication statusPublished - Jan 1 1992

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Uronic Acids
Fusarium
Chromatography
Gel Chromatography
Polysaccharides
Glycoproteins
Gels
Oligosaccharides
Hydrolysis
Nuclear magnetic resonance
Borohydrides
Acids
Methylation
Ultracentrifugation
Spectrometry
Mass spectrometry
Mass Spectrometry
Spectrum Analysis
Molecular Weight
Molecular weight

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1 : III. The primary structures of the acidic polysaccharides of the glycoproteins. / Jikibara, Takayuki; Takegawa, Kaoru; Iwahara, Shojiro.

In: Journal of biochemistry, Vol. 111, No. 2, 01.01.1992, p. 236-243.

Research output: Contribution to journalArticle

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