Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1: II. The primary structures of the low molecular weight carbohydrate chains of the glycoproteins

Takayuki Jikibara, Keiko Tada, Kaoru Takegawa, Shojiro Iwahara

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The primary structures of the O-glycosidically linked oligosaccharides isolated from glycoproteins GP I and G PII of Fusarium sp. M7-1 were established. The oligosaccharides released by alkaline borohydride treatment from the glycoproteins were purified by Bio-Gel P-4 and HPLC. This approach resulted in one monosaccharide and seven oligosaccharides. Their primary structures were resolved mainly by NMR spectrometry in combination with methylation mass spectrometry and fast atom bombardment mass spectrometry. The following structures have been determined.

Original languageEnglish
Pages (from-to)230-235
Number of pages6
JournalJournal of biochemistry
Volume111
Issue number2
DOIs
Publication statusPublished - Jan 1 1992
Externally publishedYes

Fingerprint

Uronic Acids
Fusarium
Oligosaccharides
Glycoproteins
Molecular Weight
Molecular weight
Carbohydrates
Mass spectrometry
Fast Atom Bombardment Mass Spectrometry
Borohydrides
Methylation
Monosaccharides
Spectrometry
Mass Spectrometry
Spectrum Analysis
High Pressure Liquid Chromatography
Nuclear magnetic resonance
Atoms

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Studies on the uronic acid-containing glycoproteins of Fusarium sp. M7-1 : II. The primary structures of the low molecular weight carbohydrate chains of the glycoproteins. / Jikibara, Takayuki; Tada, Keiko; Takegawa, Kaoru; Iwahara, Shojiro.

In: Journal of biochemistry, Vol. 111, No. 2, 01.01.1992, p. 230-235.

Research output: Contribution to journalArticle

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