Study on measurement of nanoparticles size based on fluorescence polarization (1st Report) - Development of rotational diffusion coefficient measurement system using fluorescent DNA probe - Development o

Yuki Ishizaki, Terutake Hayashi, Masaki Michihata, Yasuhiro Takaya

Research output: Contribution to journalArticle

Abstract

Nanoparticles are attractive materials in the area of nanotechnology, and there is a need to measure the particle size accurately and easily. We suggest a novel particle sizing method based on fluorescence polarization. Particle size evaluation can be achieved from measuring the rotational diffusion coefficient which is sensitive to the particle size. We develop the rotational diffusion coefficient measurement system using fluorescent DNA probe. We verify the feasibility of proposed method by a fundamental experiment. We measure the rotational diffusion coefficient of gold nanoparticle whose diameter is 8.2nm. The rotational diffusion coefficient decreases by 50% compared to the reference value in the measuring range. The result indicates that nanoparticle, whose diameter is as small as 8.2nm, can be measured.

Original languageEnglish
Pages (from-to)214-219
Number of pages6
JournalSeimitsu Kogaku Kaishi/Journal of the Japan Society for Precision Engineering
Volume80
Issue number2
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

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DNA
Fluorescence
Polarization
Nanoparticles
Particle size
Nanotechnology
Gold
Experiments

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering

Cite this

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