Sub-millisecond separation of DNA and micro-RNA by nanopillar array chips

Qiong Wu, Koki Motoyama, Takao Yasui, Sakon Rahong, Takeshi Yanagida, Masaki Kanai, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we fabricated a novel nanostructure that combined the pillar structures (nanopillar) and dammed structures (nanoslit) in nanometer-scale inside microchannels to realize ultra-fast separation. Nanopilliar chips were fabricated by using electron-beam lithography, photolithography and plasma etching. We used the nanopillar chip to realize ultra-fast separation of T4 DNA (165.5 kbp) and microRNA (22 b). By decreasing height down to 100 nm, we succeeded in separating DNA and microRNA in sub-milliseconds.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages599-601
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - Jan 1 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

Fingerprint

RNA
MicroRNAs
DNA
Plasma etching
Electron beam lithography
Photolithography
Microchannels
Nanostructures

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Wu, Q., Motoyama, K., Yasui, T., Rahong, S., Yanagida, T., Kanai, M., ... Baba, Y. (2012). Sub-millisecond separation of DNA and micro-RNA by nanopillar array chips. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 599-601). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.

Sub-millisecond separation of DNA and micro-RNA by nanopillar array chips. / Wu, Qiong; Motoyama, Koki; Yasui, Takao; Rahong, Sakon; Yanagida, Takeshi; Kanai, Masaki; Okamoto, Yukihiro; Kaji, Noritada; Tokeshi, Manabu; Nagashima, Kazuki; Kawai, Tomoji; Baba, Yoshinobu.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 599-601 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wu, Q, Motoyama, K, Yasui, T, Rahong, S, Yanagida, T, Kanai, M, Okamoto, Y, Kaji, N, Tokeshi, M, Nagashima, K, Kawai, T & Baba, Y 2012, Sub-millisecond separation of DNA and micro-RNA by nanopillar array chips. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Chemical and Biological Microsystems Society, pp. 599-601, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 10/28/12.
Wu Q, Motoyama K, Yasui T, Rahong S, Yanagida T, Kanai M et al. Sub-millisecond separation of DNA and micro-RNA by nanopillar array chips. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 599-601. (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).
Wu, Qiong ; Motoyama, Koki ; Yasui, Takao ; Rahong, Sakon ; Yanagida, Takeshi ; Kanai, Masaki ; Okamoto, Yukihiro ; Kaji, Noritada ; Tokeshi, Manabu ; Nagashima, Kazuki ; Kawai, Tomoji ; Baba, Yoshinobu. / Sub-millisecond separation of DNA and micro-RNA by nanopillar array chips. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 599-601 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).
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