Subcellular distribution of cytokeratin and vimentin in malignant rhabdoid tumor: Three-dimensional imaging with confocal laser scanning microscopy and double immunofluorescence

Eijun Itakura, Sadafumi Tamiya, Keisuke Morita, Hideki Shiratsuchi, Yoshiaki Kinoshita, Yumi Oshiro, Yoshinao Oda, Shigeru Ohta, Masutaka Furue, Masazumi Tsuneyoshi

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Abstract

Malignant rhabdoid tumor (MRT) is a highly aggressive neoplasm that mostly occurs in childhood, characterized histologically by rhabdoid cells as shown by eosinophilic intracytoplasmic inclusions. Although it is known that rhabdoid cells co-express cytokeratin (CK) and vimentin, the distribution patterns of these two kinds of intermediate filaments and structural relationship between them are still not known. We investigated the subcellular distribution of CKs 8 and 18 and vimentin in MRT cell lines (Tm87-16, STM91-01, TRC549, and TC289) using confocal laser scanning microscopy and double immunofluorescence, in addition to ultrastructural examination. Vimentin was diffusely expressed in the cytoplasm of MRT cells, focally forming a filamentous network. In contrast, CKs 8 and 18 were partially expressed in the cytoplasm of MRT cells, forming globules or a few vague agglomerates. Three-dimensional images in TC2B9 cells revealed distinct distribution patterns of cytokeratin and vimentin, showing agglomerates of cytokeratins within the vimentin filament network. We conclude that these globules and agglomerates of CKs 8 and 18 correspond with the characteristic ultrastructural finding, showing cytoplasmic bundles of intermediate filaments concentrated in whorled arrays.

Original languageEnglish
Pages (from-to)854-861
Number of pages8
JournalModern Pathology
Volume14
Issue number9
DOIs
Publication statusPublished - Oct 3 2001

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Rhabdoid Tumor
Three-Dimensional Imaging
Vimentin
Keratins
Confocal Microscopy
Fluorescent Antibody Technique
Intermediate Filaments
Cytoplasm
Tumor Cell Line
Neoplasms

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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Subcellular distribution of cytokeratin and vimentin in malignant rhabdoid tumor : Three-dimensional imaging with confocal laser scanning microscopy and double immunofluorescence. / Itakura, Eijun; Tamiya, Sadafumi; Morita, Keisuke; Shiratsuchi, Hideki; Kinoshita, Yoshiaki; Oshiro, Yumi; Oda, Yoshinao; Ohta, Shigeru; Furue, Masutaka; Tsuneyoshi, Masazumi.

In: Modern Pathology, Vol. 14, No. 9, 03.10.2001, p. 854-861.

Research output: Contribution to journalArticle

Itakura, Eijun ; Tamiya, Sadafumi ; Morita, Keisuke ; Shiratsuchi, Hideki ; Kinoshita, Yoshiaki ; Oshiro, Yumi ; Oda, Yoshinao ; Ohta, Shigeru ; Furue, Masutaka ; Tsuneyoshi, Masazumi. / Subcellular distribution of cytokeratin and vimentin in malignant rhabdoid tumor : Three-dimensional imaging with confocal laser scanning microscopy and double immunofluorescence. In: Modern Pathology. 2001 ; Vol. 14, No. 9. pp. 854-861.
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