Subgingival microflora and antibody responses against periodontal bacteria of young Japanese patients with type 1 diabetes mellitus.

K. Takahashi, Fusanori Nishimura, M. Kurihara, Y. Iwamoto, S. Takashiba, T. Miyata, Y. Murayama

Research output: Contribution to journalArticle

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Abstract

Periodontal disease is a complication of patients with type 1 diabetes mellitus (T1DM), although the mechanisms responsible for this relationship remain unclear. The aim of this study was to examine oral manifestations and the prevalence of periodontal pathogens from subgingival plaque samples and serum IgG antibody levels against them in young Japanese type 1 diabetic subjects. One hundred and seventeen Japanese T1DM subjects (53 male, 64 female, mean age +/- SD, 16 +/- 6.5 years) participated in this study. Thirty-nine periodontally healthy, age-matched nondiabetics served as controls. T1DM subjects were clinically assigned into three groups: 12 periodontitis, 32 gingivitis and 73 periodontally healthy. Microbiological tests for four periodontal pathogens, Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Prevotella intermedia and Capnocytophaga ochracea were performed using 16S ribosomal RNA-based polymerase chain reaction methods. Serum IgG antibody levels against 12 periodontal bacteria including the four species assessed by polymerase chain reaction were measured by enzyme-linked immunosorbent assay. In the T1DM subjects, the Periodontitis group had a significantly longer mean duration of diabetes and a higher percentages of subjects harbouring P. gingivalis and P. intermedia than the Periodontally Healthy group. Serum IgG antibody levels against P. gingivalis were significantly elevated in the Periodontitis group compared with Gingivitis and Periodontally Healthy groups. These results indicate that Japanese T1DM subjects are a high-risk group for periodontal disease and both P. gingivalis infection and duration of T1DM are risk factors for the progression of periodontitis in patients with T1DM.

Original languageEnglish
Pages (from-to)104-111
Number of pages8
JournalJournal of the International Academy of Periodontology
Volume3
Issue number4
Publication statusPublished - Jan 1 2001
Externally publishedYes

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Type 1 Diabetes Mellitus
Antibody Formation
Porphyromonas gingivalis
Bacteria
Periodontitis
Prevotella intermedia
Gingivitis
Immunoglobulin G
Periodontal Diseases
Antibodies
Serum
Capnocytophaga
16S Ribosomal RNA
Oral Manifestations
Aggregatibacter actinomycetemcomitans
Polymerase Chain Reaction
DNA-Directed RNA Polymerases
Enzyme-Linked Immunosorbent Assay
Infection

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Subgingival microflora and antibody responses against periodontal bacteria of young Japanese patients with type 1 diabetes mellitus. / Takahashi, K.; Nishimura, Fusanori; Kurihara, M.; Iwamoto, Y.; Takashiba, S.; Miyata, T.; Murayama, Y.

In: Journal of the International Academy of Periodontology, Vol. 3, No. 4, 01.01.2001, p. 104-111.

Research output: Contribution to journalArticle

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