Submerged culture conditions for mycelial yield and polysaccharides production by Lyophyllum decastes

Chandra P. Pokhrel, Shoji Ohga

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The effects of various carbon and nitrogen sources, their concentrations, initial pH and fermentation duration on the production of mycelia in terms of dry weight, exo-polysaccharide (EPS) and inner polysaccharide (IPS) by Lyophyllum decastes, a culinary-medicinal mushroom, were investigated in shake-flask cultures. Lactose, glucose and fructose were the top three best carbon sources for mycelial growth with corresponding yields of 6.73 g/l, 6.36 g/l and 6.10 g/l, respectively. Glucose was the best for production of EPS and IPS with 1.65 g/l and 317 mg/g dry mycelia, respectively. Maltose also performed well for EPS production. Yeast extract was the best nitrogen source for the production of mycelia (7.03 g/l) and IPS (325 mg/g dry mycelia), whereas EPS was improved further by increasing the yeast extract concentration (2.46 g/l at 2%). Similarly, initial pH 7 and 8 were best for polysaccharides production (EPS 1.73 g/l and IPS 320 mg/g) and mycelial growth (7.10 g/l), respectively. Maximum mycelial growth peaked at 15 days of cultivation whereas polysaccharides peaked at 10 days, and then tapered off. A concentration of glucose 3% and yeast extract 1% (mycelial yield and IPS) were found to be a suitable condition for submerged culture.

Original languageEnglish
Pages (from-to)641-646
Number of pages6
JournalFood Chemistry
Volume105
Issue number2
DOIs
Publication statusPublished - Jul 16 2007

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Lyophyllum
Polysaccharides
polysaccharides
Mycelium
mycelium
yeast extract
Yeast
Yeasts
Glucose
glucose
Nitrogen
Carbon
Growth
Batch Cell Culture Techniques
Maltose
carbon
Agaricales
nitrogen
Lactose
maltose

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Food Science
  • Medicine(all)

Cite this

Submerged culture conditions for mycelial yield and polysaccharides production by Lyophyllum decastes. / Pokhrel, Chandra P.; Ohga, Shoji.

In: Food Chemistry, Vol. 105, No. 2, 16.07.2007, p. 641-646.

Research output: Contribution to journalArticle

Pokhrel, Chandra P. ; Ohga, Shoji. / Submerged culture conditions for mycelial yield and polysaccharides production by Lyophyllum decastes. In: Food Chemistry. 2007 ; Vol. 105, No. 2. pp. 641-646.
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