Submicron resolution computed tomography for living single-cells

T. Yasokawa, I. Ishimaru, K. Ishizaki, M. Yoshida, S. Kuriyama, T. Masaki, S. Nakai, Kaoru Takegawa, N. Tanaka

Research output: Contribution to conferencePaper

1 Citation (Scopus)

Abstract

We propose the application of spectroscopy-tomography to single cells to improve the early detection and treatment of cancer. This technology can obtain the 3-dimensional distribution of components at a high spatial resolution. In this paper, we discuss the analytical results of cross-sectional imaging of a microsphere 10 μm in diameter. The distribution of the internal submicron-sized defect in the microsphere could be analyzed. To correct the center of rotation, we propose image processing using a normalized correlation function as an estimation value. The cross-sectional image of the microsphere was improved and the vague internal defect becames more clearly distinguishable by this proposed method. We also discuss variable phase-contrast spectrometry as our proposed 2-dimensional high-spatial-resolution spectrometry. This method is a phase-shift interferometry between 0th-order diffracted light and higher-order diffracted light. We discuss the experimental results of spectra derived from this method. We measured the spectral characteristics at each pixel using a color filter for liquid crystal and verified that the 2-dimensional spectral characteristics can be measured with good results.

Original languageEnglish
Pages3762-3766
Number of pages5
Publication statusPublished - Dec 1 2005
EventSICE Annual Conference 2005 - Okayama, Japan
Duration: Aug 8 2005Aug 10 2005

Other

OtherSICE Annual Conference 2005
CountryJapan
CityOkayama
Period8/8/058/10/05

Fingerprint

Microspheres
Tomography
Spectrometry
Defects
Interferometry
Phase shift
Liquid crystals
Image processing
Pixels
Spectroscopy
Color
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Computer Science Applications
  • Electrical and Electronic Engineering

Cite this

Yasokawa, T., Ishimaru, I., Ishizaki, K., Yoshida, M., Kuriyama, S., Masaki, T., ... Tanaka, N. (2005). Submicron resolution computed tomography for living single-cells. 3762-3766. Paper presented at SICE Annual Conference 2005, Okayama, Japan.

Submicron resolution computed tomography for living single-cells. / Yasokawa, T.; Ishimaru, I.; Ishizaki, K.; Yoshida, M.; Kuriyama, S.; Masaki, T.; Nakai, S.; Takegawa, Kaoru; Tanaka, N.

2005. 3762-3766 Paper presented at SICE Annual Conference 2005, Okayama, Japan.

Research output: Contribution to conferencePaper

Yasokawa, T, Ishimaru, I, Ishizaki, K, Yoshida, M, Kuriyama, S, Masaki, T, Nakai, S, Takegawa, K & Tanaka, N 2005, 'Submicron resolution computed tomography for living single-cells' Paper presented at SICE Annual Conference 2005, Okayama, Japan, 8/8/05 - 8/10/05, pp. 3762-3766.
Yasokawa T, Ishimaru I, Ishizaki K, Yoshida M, Kuriyama S, Masaki T et al. Submicron resolution computed tomography for living single-cells. 2005. Paper presented at SICE Annual Conference 2005, Okayama, Japan.
Yasokawa, T. ; Ishimaru, I. ; Ishizaki, K. ; Yoshida, M. ; Kuriyama, S. ; Masaki, T. ; Nakai, S. ; Takegawa, Kaoru ; Tanaka, N. / Submicron resolution computed tomography for living single-cells. Paper presented at SICE Annual Conference 2005, Okayama, Japan.5 p.
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AU - Yasokawa, T.

AU - Ishimaru, I.

AU - Ishizaki, K.

AU - Yoshida, M.

AU - Kuriyama, S.

AU - Masaki, T.

AU - Nakai, S.

AU - Takegawa, Kaoru

AU - Tanaka, N.

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