Submicron resolution computed tomography for living single-cells

T. Yasokawa, I. Ishimaru, K. Ishizaki, M. Yoshida, S. Kuriyama, T. Masaki, S. Nakai, K. Takegawa, N. Tanaka

Research output: Contribution to conferencePaperpeer-review

1 Citation (Scopus)

Abstract

We propose the application of spectroscopy-tomography to single cells to improve the early detection and treatment of cancer. This technology can obtain the 3-dimensional distribution of components at a high spatial resolution. In this paper, we discuss the analytical results of cross-sectional imaging of a microsphere 10 μm in diameter. The distribution of the internal submicron-sized defect in the microsphere could be analyzed. To correct the center of rotation, we propose image processing using a normalized correlation function as an estimation value. The cross-sectional image of the microsphere was improved and the vague internal defect becames more clearly distinguishable by this proposed method. We also discuss variable phase-contrast spectrometry as our proposed 2-dimensional high-spatial-resolution spectrometry. This method is a phase-shift interferometry between 0th-order diffracted light and higher-order diffracted light. We discuss the experimental results of spectra derived from this method. We measured the spectral characteristics at each pixel using a color filter for liquid crystal and verified that the 2-dimensional spectral characteristics can be measured with good results.

Original languageEnglish
Pages3762-3766
Number of pages5
Publication statusPublished - 2005
Externally publishedYes
EventSICE Annual Conference 2005 - Okayama, Japan
Duration: Aug 8 2005Aug 10 2005

Other

OtherSICE Annual Conference 2005
CountryJapan
CityOkayama
Period8/8/058/10/05

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Computer Science Applications
  • Electrical and Electronic Engineering

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