Subpopulations of CD4+ cells in lpr/lpr mice: differences in expression of T cell receptor/CD3 complex and proliferative responses

T. Asano, Yasunobu Yoshikai, K. Matsumoto, G. Matuzaki, K. Nomoto

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    Abstract

    CD4+ cells from autoimmune-prone C57BL/6 lpr/lpr mice contain two subpopulations, B220-CD4+ and B220+CD4+ cells. Highly purified B220-CD4+ cells from C57BL/6 +/+ and lpr/lpr mice were examined by comparing functional characteristics and expression of cell surface antigens and T cell receptor (TcR)/CD3 complex. Both lpr B220+CD4+ and B220+CD4-CD8- cells, most of which were PgP-1 positive, expressed TcR/CD3 complex on the cell surface at lower level as compared with B220-CD4+ cells of age-matched normal mice. In addition, the B220-CD4+ cells were heterogeneous on the basis of surface expression of PgP-1 and CD3 antigens. Normal levels of TcR C(α)-, C(β)- and V(β8)-specific mRNA were found in the B220-CD4+ cells and B220+CD4+ cells as compared with normal B220-CD4+ cells, while V(β8)-specific mRNA was preferentially expressed only by B220+CD4-CD8- cells. Either B220+CD4+ cells and B220+CD4-CD8- cells failed to respond to anti-CD3 monoclonal antibody (MoAb) as assessed by proliferative responses and production of interleukin-2 (IL-2). However, appreciable levels of reactivity to anti-CD3 MoAb were detected in the B220-CD4+ cells, although the responsiveness of this subset to such stimuli were reduced, compared with those of normal control. These results indicate that the B220-CD4+ cells in lpr mice are phenotypically and functionally distinct from normal B220-CD4+ cells.

    Original languageEnglish
    Pages (from-to)90-96
    Number of pages7
    JournalClinical and Experimental Immunology
    Volume81
    Issue number1
    Publication statusPublished - Jan 1 1990

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    CD3 Antigens
    T-Cell Antigen Receptor
    Monoclonal Antibodies
    Messenger RNA

    All Science Journal Classification (ASJC) codes

    • Immunology and Allergy
    • Immunology

    Cite this

    Subpopulations of CD4+ cells in lpr/lpr mice : differences in expression of T cell receptor/CD3 complex and proliferative responses. / Asano, T.; Yoshikai, Yasunobu; Matsumoto, K.; Matuzaki, G.; Nomoto, K.

    In: Clinical and Experimental Immunology, Vol. 81, No. 1, 01.01.1990, p. 90-96.

    Research output: Contribution to journalArticle

    Asano, T. ; Yoshikai, Yasunobu ; Matsumoto, K. ; Matuzaki, G. ; Nomoto, K. / Subpopulations of CD4+ cells in lpr/lpr mice : differences in expression of T cell receptor/CD3 complex and proliferative responses. In: Clinical and Experimental Immunology. 1990 ; Vol. 81, No. 1. pp. 90-96.
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    abstract = "CD4+ cells from autoimmune-prone C57BL/6 lpr/lpr mice contain two subpopulations, B220-CD4+ and B220+CD4+ cells. Highly purified B220-CD4+ cells from C57BL/6 +/+ and lpr/lpr mice were examined by comparing functional characteristics and expression of cell surface antigens and T cell receptor (TcR)/CD3 complex. Both lpr B220+CD4+ and B220+CD4-CD8- cells, most of which were PgP-1 positive, expressed TcR/CD3 complex on the cell surface at lower level as compared with B220-CD4+ cells of age-matched normal mice. In addition, the B220-CD4+ cells were heterogeneous on the basis of surface expression of PgP-1 and CD3 antigens. Normal levels of TcR C(α)-, C(β)- and V(β8)-specific mRNA were found in the B220-CD4+ cells and B220+CD4+ cells as compared with normal B220-CD4+ cells, while V(β8)-specific mRNA was preferentially expressed only by B220+CD4-CD8- cells. Either B220+CD4+ cells and B220+CD4-CD8- cells failed to respond to anti-CD3 monoclonal antibody (MoAb) as assessed by proliferative responses and production of interleukin-2 (IL-2). However, appreciable levels of reactivity to anti-CD3 MoAb were detected in the B220-CD4+ cells, although the responsiveness of this subset to such stimuli were reduced, compared with those of normal control. These results indicate that the B220-CD4+ cells in lpr mice are phenotypically and functionally distinct from normal B220-CD4+ cells.",
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