Expressions for apparent kinetic parameters Vappm ane Kappm observed in immobilized enzyme reactions with diffusional and electrostatic effects are proposed on the basis of the equation of a tangent to the curve of a Sb/υ versus Sb plot. The validity of these expressions is shown by comparing the expression for Kappm with two approximate expressions proposed by other workers for the reaction catalyzed by an enzyme immobilized on the external surface of a nonporous support. Calculation showed that the effect of substrate concentration on Vappm and Kappm is basically different for systems where an enzyme is immobilized on the external and pore surfaces respectively of supports.
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)