Substrates for human pluripotent stem cell cultures in conditioned medium of mesenchymal stem cells

Yusuke Ueda, Satoshi Fujita, Tatsuya Nishigaki, Yusuke Arima, Hiroo Iwata

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We aimed to establish a culture system of human pluripotent stem cells (hPSCs), such as human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), free from xenogeneic proteins, Matrigel and conditioned medium of mouse embryonic fibroblasts. The conditioned culture medium consisted of mesenchymal stem cells derived from human bone marrow. We examined surface properties suitable for hPSC culture by using self-assembled monolayers (SAMs) of alkanethiols with four different functional groups: CH3, OH, COOH and NH2. hPSCs neither adhered nor proliferated on surfaces with a water contact angle higher than 40°. Based on this finding, the contact angle of a polystyrene (PSt) culture dish was reduced to less than 40°, and COOH and OH groups were introduced to its surface by oxygen plasma treatment, making the PSt dish suitable for hPSC culture. This combination of a PSt dish treated with oxygen plasma treatment and conditioned medium of mesenchymal stem cells achieved a long-term maintenance of hPSCs without differentiation.

Original languageEnglish
Pages (from-to)153-165
Number of pages13
JournalJournal of Biomaterials Science, Polymer Edition
Volume23
Issue number1-4
DOIs
Publication statusPublished - Jan 1 2012

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Pluripotent Stem Cells
Conditioned Culture Medium
Stem cells
Mesenchymal Stromal Cells
Cell culture
Cell Culture Techniques
Polystyrenes
Substrates
Oxygen
Induced Pluripotent Stem Cells
Contact angle
Surface Properties
Plasmas
Cell Differentiation
Fibroblasts
Bone Marrow
Self assembled monolayers
Functional groups
Surface properties
Water

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

Cite this

Substrates for human pluripotent stem cell cultures in conditioned medium of mesenchymal stem cells. / Ueda, Yusuke; Fujita, Satoshi; Nishigaki, Tatsuya; Arima, Yusuke; Iwata, Hiroo.

In: Journal of Biomaterials Science, Polymer Edition, Vol. 23, No. 1-4, 01.01.2012, p. 153-165.

Research output: Contribution to journalArticle

Ueda, Yusuke ; Fujita, Satoshi ; Nishigaki, Tatsuya ; Arima, Yusuke ; Iwata, Hiroo. / Substrates for human pluripotent stem cell cultures in conditioned medium of mesenchymal stem cells. In: Journal of Biomaterials Science, Polymer Edition. 2012 ; Vol. 23, No. 1-4. pp. 153-165.
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