TY - JOUR
T1 - Substrates for human pluripotent stem cell cultures in conditioned medium of mesenchymal stem cells
AU - Ueda, Yusuke
AU - Fujita, Satoshi
AU - Nishigaki, Tatsuya
AU - Arima, Yusuke
AU - Iwata, Hiroo
N1 - Funding Information:
We acknowledge Dr. H. Suemori for providing hESCs and Dr. T. Aoyama for providing immortalized MSC. This study was supported in part by the Collaborative Development of Innovative Seeds of the Japan Science and Technology Agency (JST).
PY - 2012
Y1 - 2012
N2 - We aimed to establish a culture system of human pluripotent stem cells (hPSCs), such as human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), free from xenogeneic proteins, Matrigel ™ and conditioned medium of mouse embryonic fibroblasts. The conditioned culture medium consisted of mesenchymal stem cells derived from human bone marrow. We examined surface properties suitable for hPSC culture by using self-assembled monolayers (SAMs) of alkanethiols with four different functional groups: CH3, OH, COOH and NH2. hPSCs neither adhered nor proliferated on surfaces with a water contact angle higher than 40°. Based on this finding, the contact angle of a polystyrene (PSt) culture dish was reduced to less than 40°, and COOH and OH groups were introduced to its surface by oxygen plasma treatment, making the PSt dish suitable for hPSC culture. This combination of a PSt dish treated with oxygen plasma treatment and conditioned medium of mesenchymal stem cells achieved a long-term maintenance of hPSCs without differentiation.
AB - We aimed to establish a culture system of human pluripotent stem cells (hPSCs), such as human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), free from xenogeneic proteins, Matrigel ™ and conditioned medium of mouse embryonic fibroblasts. The conditioned culture medium consisted of mesenchymal stem cells derived from human bone marrow. We examined surface properties suitable for hPSC culture by using self-assembled monolayers (SAMs) of alkanethiols with four different functional groups: CH3, OH, COOH and NH2. hPSCs neither adhered nor proliferated on surfaces with a water contact angle higher than 40°. Based on this finding, the contact angle of a polystyrene (PSt) culture dish was reduced to less than 40°, and COOH and OH groups were introduced to its surface by oxygen plasma treatment, making the PSt dish suitable for hPSC culture. This combination of a PSt dish treated with oxygen plasma treatment and conditioned medium of mesenchymal stem cells achieved a long-term maintenance of hPSCs without differentiation.
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U2 - 10.1163/092050610X545797
DO - 10.1163/092050610X545797
M3 - Article
C2 - 22133351
AN - SCOPUS:82955189269
SN - 0920-5063
VL - 23
SP - 153
EP - 165
JO - Journal of Biomaterials Science, Polymer Edition
JF - Journal of Biomaterials Science, Polymer Edition
IS - 1-4
ER -