Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion and NG108-15 cells

Haruhiro Higashida, Jia Sheng Zhang, Sumiko Mochida, Xiao Liang Chen, Yeonsook Shin, Mami Noda, Kazi Z. Hossain, Naoto Hoshi, Minako Hashii, Ryuichi Shigemoto, Shigetada Nakanishi, Yutaka Fukuda, Shigeru Yokoyama

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Cyclic ADP-ribose (cADP-ribose) is a putative second messenger or modulator. However, the role of cADP-ribose in the downstream signals of the metabotropic glutamate receptors (mGluRs) is unclear. Here, we show that glutamate stimulates ADP-ribosyl cyclase activity in rat or mouse crude membranes of retina via group III mGluRs or in superior cervical ganglion via group I mGluRs. The retina of mGluR6-deficient mice showed no increase in the ADP-ribosyl cyclase level in response to glutamate. GTP enhanced the initial rate of basal and glutamate-stimulated cyclase activity. GTP-γ-S also stimulated basal activity. To determine whether the coupling mode of mGluRs to ADP-ribosyl cyclase is a feature common to individual cloned mGluRs, we expressed each mGluR subtype in NG108-15 neuroblastoma x glioma hybrid cells. The glutamate-induced stimulation of the cyclase occurs preferentially in NG108-15 cells over-expressing mGluRs1, 3, 5, and 6. Cells expressing mGluR2 or mGluRs4 and 7 exhibit inhibition or no coupling, respectively. Glutamate-induced activation or inhibition of the cyclase activity was eliminated after pre-treatment with cholera or pertussis toxin, respectively. Thus, the subtype-specific coupling of mGluRs to ADP-ribosyl cyclase via G proteins suggests that some glutamate-evoked neuronal functions are mediated by cADP-ribose.

Original languageEnglish
Pages (from-to)1148-1158
Number of pages11
JournalJournal of Neurochemistry
Volume85
Issue number5
DOIs
Publication statusPublished - Jun 1 2003

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ADP-ribosyl Cyclase
Superior Cervical Ganglion
Metabotropic Glutamate Receptors
Retina
Glutamic Acid
Cyclic ADP-Ribose
Guanosine Triphosphate
Hybrid Cells
Cholera Toxin
Pertussis Toxin
Second Messenger Systems
Neuroblastoma
GTP-Binding Proteins
Glioma
Modulators
Rats
Chemical activation
Cells
Membranes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion and NG108-15 cells. / Higashida, Haruhiro; Zhang, Jia Sheng; Mochida, Sumiko; Chen, Xiao Liang; Shin, Yeonsook; Noda, Mami; Hossain, Kazi Z.; Hoshi, Naoto; Hashii, Minako; Shigemoto, Ryuichi; Nakanishi, Shigetada; Fukuda, Yutaka; Yokoyama, Shigeru.

In: Journal of Neurochemistry, Vol. 85, No. 5, 01.06.2003, p. 1148-1158.

Research output: Contribution to journalArticle

Higashida, H, Zhang, JS, Mochida, S, Chen, XL, Shin, Y, Noda, M, Hossain, KZ, Hoshi, N, Hashii, M, Shigemoto, R, Nakanishi, S, Fukuda, Y & Yokoyama, S 2003, 'Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion and NG108-15 cells', Journal of Neurochemistry, vol. 85, no. 5, pp. 1148-1158. https://doi.org/10.1046/j.1471-4159.2003.01751.x
Higashida, Haruhiro ; Zhang, Jia Sheng ; Mochida, Sumiko ; Chen, Xiao Liang ; Shin, Yeonsook ; Noda, Mami ; Hossain, Kazi Z. ; Hoshi, Naoto ; Hashii, Minako ; Shigemoto, Ryuichi ; Nakanishi, Shigetada ; Fukuda, Yutaka ; Yokoyama, Shigeru. / Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion and NG108-15 cells. In: Journal of Neurochemistry. 2003 ; Vol. 85, No. 5. pp. 1148-1158.
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AU - Hossain, Kazi Z.

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