Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion and NG108-15 cells

Haruhiro Higashida, Jia Sheng Zhang, Sumiko Mochida, Xiao Liang Chen, Yeonsook Shin, Mami Noda, Kazi Z. Hossain, Naoto Hoshi, Minako Hashii, Ryuichi Shigemoto, Shigetada Nakanishi, Yutaka Fukuda, Shigeru Yokoyama

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17 Citations (Scopus)

Abstract

Cyclic ADP-ribose (cADP-ribose) is a putative second messenger or modulator. However, the role of cADP-ribose in the downstream signals of the metabotropic glutamate receptors (mGluRs) is unclear. Here, we show that glutamate stimulates ADP-ribosyl cyclase activity in rat or mouse crude membranes of retina via group III mGluRs or in superior cervical ganglion via group I mGluRs. The retina of mGluR6-deficient mice showed no increase in the ADP-ribosyl cyclase level in response to glutamate. GTP enhanced the initial rate of basal and glutamate-stimulated cyclase activity. GTP-γ-S also stimulated basal activity. To determine whether the coupling mode of mGluRs to ADP-ribosyl cyclase is a feature common to individual cloned mGluRs, we expressed each mGluR subtype in NG108-15 neuroblastoma x glioma hybrid cells. The glutamate-induced stimulation of the cyclase occurs preferentially in NG108-15 cells over-expressing mGluRs1, 3, 5, and 6. Cells expressing mGluR2 or mGluRs4 and 7 exhibit inhibition or no coupling, respectively. Glutamate-induced activation or inhibition of the cyclase activity was eliminated after pre-treatment with cholera or pertussis toxin, respectively. Thus, the subtype-specific coupling of mGluRs to ADP-ribosyl cyclase via G proteins suggests that some glutamate-evoked neuronal functions are mediated by cADP-ribose.

Original languageEnglish
Pages (from-to)1148-1158
Number of pages11
JournalJournal of Neurochemistry
Volume85
Issue number5
DOIs
Publication statusPublished - Jun 2003

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

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    Higashida, H., Zhang, J. S., Mochida, S., Chen, X. L., Shin, Y., Noda, M., Hossain, K. Z., Hoshi, N., Hashii, M., Shigemoto, R., Nakanishi, S., Fukuda, Y., & Yokoyama, S. (2003). Subtype-specific coupling with ADP-ribosyl cyclase of metabotropic glutamate receptors in retina, cervical superior ganglion and NG108-15 cells. Journal of Neurochemistry, 85(5), 1148-1158. https://doi.org/10.1046/j.1471-4159.2003.01751.x