Subtypes of metabotropic glutamate receptors in the nucleus of the solitary tract of rats

Kiyoshi Matsumura, Takuya Tsuchihashi, Shuntaro Kagiyama, Isao Abe, Masatoshi Fujishima

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Abstract

We have determined the role of subgroups of metabotropic glutamate receptors (mGluRs) in the nucleus of the solitary tract (NTS) of normotensive Wistar rats. Unilateral microinjection of (S)-3,5-dihydroxyphenylglycine (3,5-DHPG), an agonist of group I mGluRs, into the NTS significantly decreased mean arterial pressure (MAP), heart rate (HR), and renal sympathetic nerve activity (RSNA) (-19.4 ± 2.6 mmHg, -16.4 ± 5.1 beats/min, and -30.6 ± 5.7% by 1 nmol). Microinjection of (R,S)-1-aminoindan-1,5- dicarboxylic acid (AIDA; 1 nmol), a putative antagonist of group I mGluRs, into the NTS caused transient decreases in MAP and RSNA, followed by sustained increases in MAP (+8.3 ± 2.4 mmHg) and RSNA (+27.7 ± 10.8%). Pretreatment with AIDA failed to prevent the cardiovascular and RSNA responses to microinjection of 3,5-DHPG. Unilateral microinjection of (S)-4- carboxy-3-hydroxyphenylglycine (4C3HPG), an agonist of group II mGluRs, into the NTS also significantly decreased MAP, HR, and RSNA, whose responses were not inhibited by pre-microinjection of (2S)-α-ethylglutamic acid (EGLU; 2 nmol), a putative antagonist of group II mGluRs. On the other hand, unilateral microinjection of L(+)-2-amino-4-phosphonobutyric acid (L-AP4), an agonist of group III mGluRs, into the NTS caused dose-related decreases in MAP (-8.3 ± 1.5 mmHg by 0.1 nmol and -45.1 ± 3.4 mmHg by 0.3 nmol), HR, and RSNA (-21.3 ± 3.9% by 0.1 nmol and -77.2 ± 6.5% by 0.3 nmol), whose responses were suppressed by pre-microinjection of (R,S)-α-cyclopropyl-4- phosphonophenylglycine (CPPG; 0.3 nmol), an antagonist of group III mGluRs. These results suggest that all subgroups of mGluRs participate in cardiovascular and sympathetic regulations in the NTS of rats, and that endogenous group I mGluRs in the NTS may contribute to tonic cardiovascular and sympathetic regulations.

Original languageEnglish
Pages (from-to)461-468
Number of pages8
JournalBrain Research
Volume842
Issue number2
DOIs
Publication statusPublished - Sep 25 1999

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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    Matsumura, K., Tsuchihashi, T., Kagiyama, S., Abe, I., & Fujishima, M. (1999). Subtypes of metabotropic glutamate receptors in the nucleus of the solitary tract of rats. Brain Research, 842(2), 461-468. https://doi.org/10.1016/S0006-8993(99)01889-2