Successful and optimized in vivo gene transfer to rabbit carotid artery mediated by electronic pulse

T. Matsumoto, K. Komori, T. Shoji, S. Kuma, M. Kume, T. Yamaoka, E. Mori, T. Furuyama, Y. Yonemitsu, K. Sugimachi

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Several gene transfer methods, including viral or nonviral vehicles have been developed, however, efficacy, safety or handling continue to present problems. We developed a nonviral and plasmid-based method for arterial gene transfer by in vivo electronic pulse, using a newly designed T-shaped electrode. Using rabbit carotid arteries, we first optimized gene transfer efficiency, and firefly luciferase gene transfer via electronic pulse under 20 voltage (the pulse length: Pon time 20 ms, the pulse interval: Poff time 80 ms, number of pulse: 10 times) showed the highest gene expression. Exogenous gene expression was detectable for at least up to 14 days. Electroporation-mediated gene transfer of E. coli lacZ with nuclear localizing signal revealed successful gene transfer to luminal endothelial cells and to medial cells. Histological damage was recognized as the voltage was increased but neointima formation 4 weeks after gene transfer was not induced. In vivo electroporation-mediated arterial gene transfer is readily facilitated, is safe and may prove to be an alternative form of gene transfer to the vasculature.

Original languageEnglish
Pages (from-to)1174-1179
Number of pages6
JournalGene Therapy
Volume8
Issue number15
DOIs
Publication statusPublished - Sep 3 2001

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Molecular Biology
  • Genetics

Cite this

Matsumoto, T., Komori, K., Shoji, T., Kuma, S., Kume, M., Yamaoka, T., ... Sugimachi, K. (2001). Successful and optimized in vivo gene transfer to rabbit carotid artery mediated by electronic pulse. Gene Therapy, 8(15), 1174-1179. https://doi.org/10.1038/sj.gt.3301502