Sucrose laurate-enhanced transcutaneous immunization with a solid-in-oil nanodispersion

Momoko Kitaoka, Kana Imamura, Yuya Hirakawa, Yoshiro Tahara, Noriho Kamiya, Masahiro Goto

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A novel transcutaneous immunization system was developed using a solid-in-oil (S/O) nanodispersion, consisting of a nano-sized particle of a protein-surfactant complex dispersed in an oil vehicle. Permeability of the model antigen protein, ovalbumin, was enhanced by coating with sucrose laurate, which is a hydrophobic surfactant. The nanodispersion prepared with sucrose laurate induced a 5-fold increase in mouse antigen-specific antibody production compared with that observed using sucrose erucate, indicating that stability of the S/O nanodispersion in the epidermis plays a key role in enabling effective immunization. In spite of the coating with the surfactants, only fluorescence-labeled ovalbumin permeated the deep epidermis beneath the stratum corneum and was observed by laser scanning confocal microscopy. These findings will lead the way to an improved transcutaneous immunization system based on S/O nanodispersion.

Original languageEnglish
Pages (from-to)20-24
Number of pages5
JournalMedChemComm
Volume5
Issue number1
DOIs
Publication statusPublished - Jan 2014

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

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