Sugar microarray via click chemistry: Molecular recognition with lectins and amyloid beta; (1-42)

Erino Matsumoto, Takahiro Yamauchi, Tomohiro Fukuda, Yoshiko Miura

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Sugar microarrays were fabricated on various substrates via click chemistry. Acetylene-terminated substrates were prepared by forming self-assembled monolayers (SAMs) on a gold substrate with alkyl-disulfide and on silicon, quartz and glass substrates with a silane-coupling reagent. The gold substrates were subjected to surface plasmon resonance measurements, and the quartz and glass substrates were subjected to spectroscopy measurements and optical microscopy observation. The saccharide-immobilized substrate on the gold substrate showed specific interaction with the corresponding lectin, and the saccharides showed inert surface properties to other proteins with a high signal-to-noise ratio. We also focused on the saccharide-protein interaction on protein amyloidosis of Alzheimer amyloid β. Amyloid β peptide showed conformation transition on the saccharide-immobilization substrate into a β-sheet, and fibril formation and amyloid aggregates were found on the specific saccharides.

Original languageEnglish
Article number034605
JournalScience and Technology of Advanced Materials
Volume10
Issue number3
DOIs
Publication statusPublished - Oct 27 2009
Externally publishedYes

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Molecular recognition
Microarrays
Amyloid
Lectins
Sugars
Substrates
Gold
Quartz
Proteins
Silanes
Glass
Acetylene
Surface plasmon resonance
Self assembled monolayers
Silicon
Disulfides
Peptides
Optical microscopy
Surface properties
Conformations

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Sugar microarray via click chemistry : Molecular recognition with lectins and amyloid beta; (1-42). / Matsumoto, Erino; Yamauchi, Takahiro; Fukuda, Tomohiro; Miura, Yoshiko.

In: Science and Technology of Advanced Materials, Vol. 10, No. 3, 034605, 27.10.2009.

Research output: Contribution to journalArticle

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