Sulfate Conjugation of Various Phenols by Liver-Soluble Fraction of Goldfish

A study has been made of the capability of the sulfate conjugating enzyme system in the liver-soluble fraction from goldfish Carassius auratus, to phenol and twenty-one phenolic compounds as follows at various concentrations from 0.00067 to 33.3 mm in reaction media: (1) chlorophenols (o-;p-; 2,4-; 2,4,5-; 2,4,6-; 2,3,4,6-; 2,3,4,5,6-), (2) aminophenols (o-; p-), (3) nitrophenols (m-; o-; p-; 2,4-; 2,4,6-), (4) polyphenols (resorcinol; catechol; pyrogallol), (5) other phenols(p-cresol; o,o’- biphenol; phenolphthalein; phenol red). The liver-soluble fraction showed fairly high activities of the sulfate conjugation for half of the tested phenols corresponding to 53-116 % of that for phenol (246 nmol/h/g-liver), while the negligible conjugation for less than 5% of that for phenol was observed only in three phenols, i.e., 2,4-dinitrophenol, 2,4,6-trinitrophenol and phenol red. On the other hand, some phenols which showed low sulfate conjugation among the tested phenols such as pentachlorophenol strongly inhibited the sulfate conjugating enzyme system.