Sulfolobus tokodaii ST0053 produces a novel thermostable, NAD-dependent medium-chain alcohol dehydrogenase

Hisaaki Yanai, Katsumi Doi, Toshihisa Ohshima

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

An alcohol dehydrogenase (ADH) gene, ST0053, from Sulfolobus tokodaii was expressed In Escherichia coli. The purified recombinant enzyme was an NAD-dependent medium-chain ADH with high thermostability and tolerance of a wide range of pHs. This is the first step in creating an experimental functionality library of 10 genes annotated as ADHs in the S. tokodaii genome.

Original languageEnglish
Pages (from-to)1758-1763
Number of pages6
JournalApplied and environmental microbiology
Volume75
Issue number6
DOIs
Publication statusPublished - Mar 1 2009

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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