An alcohol dehydrogenase (ADH) gene, ST0053, from Sulfolobus tokodaii was expressed In Escherichia coli. The purified recombinant enzyme was an NAD-dependent medium-chain ADH with high thermostability and tolerance of a wide range of pHs. This is the first step in creating an experimental functionality library of 10 genes annotated as ADHs in the S. tokodaii genome.
All Science Journal Classification (ASJC) codes
- Food Science
- Applied Microbiology and Biotechnology