Sulfolobus tokodaii ST0053 produces a novel thermostable, NAD-dependent medium-chain alcohol dehydrogenase

Hisaaki Yanai; Katsumi Doi; Toshihisa Ohshima

doi:10.1128/AEM.01392-08

Sulfolobus tokodaii ST0053 produces a novel thermostable, NAD-dependent medium-chain alcohol dehydrogenase

Hisaaki Yanai, Katsumi Doi, Toshihisa Ohshima

Systems Biology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

An alcohol dehydrogenase (ADH) gene, ST0053, from Sulfolobus tokodaii was expressed In Escherichia coli. The purified recombinant enzyme was an NAD-dependent medium-chain ADH with high thermostability and tolerance of a wide range of pHs. This is the first step in creating an experimental functionality library of 10 genes annotated as ADHs in the S. tokodaii genome.

Original language	English
Pages (from-to)	1758-1763
Number of pages	6
Journal	Applied and environmental microbiology
Volume	75
Issue number	6
DOIs	https://doi.org/10.1128/AEM.01392-08
Publication status	Published - Mar 2009

All Science Journal Classification (ASJC) codes

Biotechnology
Food Science
Ecology
Applied Microbiology and Biotechnology

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abstract = "An alcohol dehydrogenase (ADH) gene, ST0053, from Sulfolobus tokodaii was expressed In Escherichia coli. The purified recombinant enzyme was an NAD-dependent medium-chain ADH with high thermostability and tolerance of a wide range of pHs. This is the first step in creating an experimental functionality library of 10 genes annotated as ADHs in the S. tokodaii genome.",

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Sulfolobus tokodaii ST0053 produces a novel thermostable, NAD-dependent medium-chain alcohol dehydrogenase

Abstract

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