Sulforaphane inhibits the growth of KPL-1 human breast cancer cells in vitro and suppresses the growth and metastasis of orthotopically transplanted KPL-1 cells in female athymic mice

Sayaka Kanematsu, Katsuhiko Yoshizawa, Norihisa Uehara, Hisanori Miki, Tomo Sasaki, Maki Kuro, Yen Chang Lai, Ayako Kimura, Takashi Yuri, Airo Tsubura

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The anticancer effects of sulforaphane (SFN), which is found in cruciferous vegetables, were studied on KPL-1 human breast cancer cells in vitro and in vivo. Cell proliferation in vitro was assessed by a 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay, and tumor growth and metastasis in vivo were examined in orthotopically (right thoracic mammary fat pad) transplanted KPL-1 cells in female athymic BALB/c mice. The MTT assay showed that SFN directly inhibited KPL-1 cell growth in vitro (IC50 at 48 h, 19.1 μM; IC50 at 72 h, 17.8 μM). Athymic mice received a KPL-1 cell transplant, and SFN treatment (intraperitoneal injection of 25 or 50 mg/kg SFN) was started the next day. Mice received five injections each week during the 26-day experimental period (for a total of 20 injections). Compared with the SFN-untreated controls, SFN suppressed primary tumor growth. At the termination of the experiment, the final tumor volume was 686±94 mm3 for the control group, 516±70 mm3 (75% of control value) for the 25 mg/kg SFN group and 351±55 mm3 (51% of control value) for the 50 mg/kg SFN group. The final tumor weight was 571±69 mg in the control group, 416±63 mg (73% of the control value) in the 25 mg/kg SFN group and 338±56 mg (59% of the control value) in the 50 mg/kg SFN group. SFN caused a dose-dependent decrease in the proliferation ratio and an increase in the apoptotic ratio of the primary tumor cells. SFN treatment tended to reduce regional (axillary) lymph node metastasis. Treatment with 50 mg/kg SFN significantly inhibited KPL-1 cell growth in vivo by suppressing cell proliferation and inducing apoptosis, and it tended to reduce axillary lymph node metastasis of KPL-1 human breast cancer cell xenografts in female athymic mice.

Original languageEnglish
Pages (from-to)603-608
Number of pages6
JournalOncology reports
Volume26
Issue number3
DOIs
Publication statusPublished - Sep 1 2011
Externally publishedYes

Fingerprint

Nude Mice
Breast Neoplasms
Neoplasm Metastasis
Growth
Tumor Burden
sulforafan
In Vitro Techniques
Inhibitory Concentration 50
Lymph Nodes
Cell Proliferation
Neoplasms
Control Groups
Injections
Intraperitoneal Injections
Heterografts
Vegetables
Adipose Tissue
Breast
Thorax
Therapeutics

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Sulforaphane inhibits the growth of KPL-1 human breast cancer cells in vitro and suppresses the growth and metastasis of orthotopically transplanted KPL-1 cells in female athymic mice. / Kanematsu, Sayaka; Yoshizawa, Katsuhiko; Uehara, Norihisa; Miki, Hisanori; Sasaki, Tomo; Kuro, Maki; Lai, Yen Chang; Kimura, Ayako; Yuri, Takashi; Tsubura, Airo.

In: Oncology reports, Vol. 26, No. 3, 01.09.2011, p. 603-608.

Research output: Contribution to journalArticle

Kanematsu, Sayaka ; Yoshizawa, Katsuhiko ; Uehara, Norihisa ; Miki, Hisanori ; Sasaki, Tomo ; Kuro, Maki ; Lai, Yen Chang ; Kimura, Ayako ; Yuri, Takashi ; Tsubura, Airo. / Sulforaphane inhibits the growth of KPL-1 human breast cancer cells in vitro and suppresses the growth and metastasis of orthotopically transplanted KPL-1 cells in female athymic mice. In: Oncology reports. 2011 ; Vol. 26, No. 3. pp. 603-608.
@article{3f37152e40b048eca9b042cefb5cd912,
title = "Sulforaphane inhibits the growth of KPL-1 human breast cancer cells in vitro and suppresses the growth and metastasis of orthotopically transplanted KPL-1 cells in female athymic mice",
abstract = "The anticancer effects of sulforaphane (SFN), which is found in cruciferous vegetables, were studied on KPL-1 human breast cancer cells in vitro and in vivo. Cell proliferation in vitro was assessed by a 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay, and tumor growth and metastasis in vivo were examined in orthotopically (right thoracic mammary fat pad) transplanted KPL-1 cells in female athymic BALB/c mice. The MTT assay showed that SFN directly inhibited KPL-1 cell growth in vitro (IC50 at 48 h, 19.1 μM; IC50 at 72 h, 17.8 μM). Athymic mice received a KPL-1 cell transplant, and SFN treatment (intraperitoneal injection of 25 or 50 mg/kg SFN) was started the next day. Mice received five injections each week during the 26-day experimental period (for a total of 20 injections). Compared with the SFN-untreated controls, SFN suppressed primary tumor growth. At the termination of the experiment, the final tumor volume was 686±94 mm3 for the control group, 516±70 mm3 (75{\%} of control value) for the 25 mg/kg SFN group and 351±55 mm3 (51{\%} of control value) for the 50 mg/kg SFN group. The final tumor weight was 571±69 mg in the control group, 416±63 mg (73{\%} of the control value) in the 25 mg/kg SFN group and 338±56 mg (59{\%} of the control value) in the 50 mg/kg SFN group. SFN caused a dose-dependent decrease in the proliferation ratio and an increase in the apoptotic ratio of the primary tumor cells. SFN treatment tended to reduce regional (axillary) lymph node metastasis. Treatment with 50 mg/kg SFN significantly inhibited KPL-1 cell growth in vivo by suppressing cell proliferation and inducing apoptosis, and it tended to reduce axillary lymph node metastasis of KPL-1 human breast cancer cell xenografts in female athymic mice.",
author = "Sayaka Kanematsu and Katsuhiko Yoshizawa and Norihisa Uehara and Hisanori Miki and Tomo Sasaki and Maki Kuro and Lai, {Yen Chang} and Ayako Kimura and Takashi Yuri and Airo Tsubura",
year = "2011",
month = "9",
day = "1",
doi = "10.3892/or.2011.1311",
language = "English",
volume = "26",
pages = "603--608",
journal = "Oncology Reports",
issn = "1021-335X",
publisher = "Spandidos Publications",
number = "3",

}

TY - JOUR

T1 - Sulforaphane inhibits the growth of KPL-1 human breast cancer cells in vitro and suppresses the growth and metastasis of orthotopically transplanted KPL-1 cells in female athymic mice

AU - Kanematsu, Sayaka

AU - Yoshizawa, Katsuhiko

AU - Uehara, Norihisa

AU - Miki, Hisanori

AU - Sasaki, Tomo

AU - Kuro, Maki

AU - Lai, Yen Chang

AU - Kimura, Ayako

AU - Yuri, Takashi

AU - Tsubura, Airo

PY - 2011/9/1

Y1 - 2011/9/1

N2 - The anticancer effects of sulforaphane (SFN), which is found in cruciferous vegetables, were studied on KPL-1 human breast cancer cells in vitro and in vivo. Cell proliferation in vitro was assessed by a 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay, and tumor growth and metastasis in vivo were examined in orthotopically (right thoracic mammary fat pad) transplanted KPL-1 cells in female athymic BALB/c mice. The MTT assay showed that SFN directly inhibited KPL-1 cell growth in vitro (IC50 at 48 h, 19.1 μM; IC50 at 72 h, 17.8 μM). Athymic mice received a KPL-1 cell transplant, and SFN treatment (intraperitoneal injection of 25 or 50 mg/kg SFN) was started the next day. Mice received five injections each week during the 26-day experimental period (for a total of 20 injections). Compared with the SFN-untreated controls, SFN suppressed primary tumor growth. At the termination of the experiment, the final tumor volume was 686±94 mm3 for the control group, 516±70 mm3 (75% of control value) for the 25 mg/kg SFN group and 351±55 mm3 (51% of control value) for the 50 mg/kg SFN group. The final tumor weight was 571±69 mg in the control group, 416±63 mg (73% of the control value) in the 25 mg/kg SFN group and 338±56 mg (59% of the control value) in the 50 mg/kg SFN group. SFN caused a dose-dependent decrease in the proliferation ratio and an increase in the apoptotic ratio of the primary tumor cells. SFN treatment tended to reduce regional (axillary) lymph node metastasis. Treatment with 50 mg/kg SFN significantly inhibited KPL-1 cell growth in vivo by suppressing cell proliferation and inducing apoptosis, and it tended to reduce axillary lymph node metastasis of KPL-1 human breast cancer cell xenografts in female athymic mice.

AB - The anticancer effects of sulforaphane (SFN), which is found in cruciferous vegetables, were studied on KPL-1 human breast cancer cells in vitro and in vivo. Cell proliferation in vitro was assessed by a 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assay, and tumor growth and metastasis in vivo were examined in orthotopically (right thoracic mammary fat pad) transplanted KPL-1 cells in female athymic BALB/c mice. The MTT assay showed that SFN directly inhibited KPL-1 cell growth in vitro (IC50 at 48 h, 19.1 μM; IC50 at 72 h, 17.8 μM). Athymic mice received a KPL-1 cell transplant, and SFN treatment (intraperitoneal injection of 25 or 50 mg/kg SFN) was started the next day. Mice received five injections each week during the 26-day experimental period (for a total of 20 injections). Compared with the SFN-untreated controls, SFN suppressed primary tumor growth. At the termination of the experiment, the final tumor volume was 686±94 mm3 for the control group, 516±70 mm3 (75% of control value) for the 25 mg/kg SFN group and 351±55 mm3 (51% of control value) for the 50 mg/kg SFN group. The final tumor weight was 571±69 mg in the control group, 416±63 mg (73% of the control value) in the 25 mg/kg SFN group and 338±56 mg (59% of the control value) in the 50 mg/kg SFN group. SFN caused a dose-dependent decrease in the proliferation ratio and an increase in the apoptotic ratio of the primary tumor cells. SFN treatment tended to reduce regional (axillary) lymph node metastasis. Treatment with 50 mg/kg SFN significantly inhibited KPL-1 cell growth in vivo by suppressing cell proliferation and inducing apoptosis, and it tended to reduce axillary lymph node metastasis of KPL-1 human breast cancer cell xenografts in female athymic mice.

UR - http://www.scopus.com/inward/record.url?scp=79959962141&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79959962141&partnerID=8YFLogxK

U2 - 10.3892/or.2011.1311

DO - 10.3892/or.2011.1311

M3 - Article

C2 - 21617865

AN - SCOPUS:79959962141

VL - 26

SP - 603

EP - 608

JO - Oncology Reports

JF - Oncology Reports

SN - 1021-335X

IS - 3

ER -