Sulphur starvation induces the expression of microRNA-395 and one of its target genes but in different cell types

Cintia Goulart Kawashima, Naoko Yoshimoto, Akiko Maruyama, Yumiko N. Tsuchiya, Kazuki Saito, Hideki Takahashi, Tamas Dalmay

Research output: Contribution to journalArticle

265 Citations (Scopus)

Abstract

Plants play an important role in the global sulphur cycle because they assimilate sulphur from the environment and build it into methionine and cysteine. Several genes of the sulphur assimilation pathway are regulated by microRNA-395 (miR395) that is itself induced by a low-sulphur (-S) environment. Here, we show that the six Arabidopsis miR395 loci are induced differently. We find that MIR395 loci are expressed in the vascular system of roots and leaves and root tips. Induction of miR395 by a -S environment in both roots and leaves suggests that translocation of miR395 from leaves to roots through the phloem is not necessary for plants growing on -S soil/medium. We also demonstrate that induction of miR395 is controlled by SLIM1, a key transcription factor in the sulphur assimilation pathway. Unexpectedly, the mRNA level of a miR395 target gene, SULTR2;1, strongly increases during miR395 induction in roots. We show that the spatial expression pattern of MIR395 transcripts in the vascular system does not appear to overlap with the expression pattern previously reported for SULTR2;1 mRNA. These results illustrate that negative temporal correlation between the expression level of a miRNA and its target gene in a complex tissue cannot be a requirement for target gene validation.

Original languageEnglish
Pages (from-to)313-321
Number of pages9
JournalPlant Journal
Volume57
Issue number2
DOIs
Publication statusPublished - Jan 1 2009

Fingerprint

Starvation
MicroRNAs
microRNA
Sulfur
starvation
sulfur
Genes
genes
cells
plant vascular system
Blood Vessels
Phloem
Messenger RNA
Meristem
leaves
loci
Methionine
Cysteine
root tips
phloem

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Sulphur starvation induces the expression of microRNA-395 and one of its target genes but in different cell types. / Kawashima, Cintia Goulart; Yoshimoto, Naoko; Maruyama, Akiko; Tsuchiya, Yumiko N.; Saito, Kazuki; Takahashi, Hideki; Dalmay, Tamas.

In: Plant Journal, Vol. 57, No. 2, 01.01.2009, p. 313-321.

Research output: Contribution to journalArticle

Kawashima, Cintia Goulart ; Yoshimoto, Naoko ; Maruyama, Akiko ; Tsuchiya, Yumiko N. ; Saito, Kazuki ; Takahashi, Hideki ; Dalmay, Tamas. / Sulphur starvation induces the expression of microRNA-395 and one of its target genes but in different cell types. In: Plant Journal. 2009 ; Vol. 57, No. 2. pp. 313-321.
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