1H-NMR Method Enables Early Identification of Degeneration in the Quality of Sweet Potato Tubers

M. Iwaya-Inoue, R. Matsui, N. Sultana, K. Saitou, K. Sakaguchi, M. Fukuyama

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In sweet potato tuber, which is a tropical plant, long-term storage leads to loss of water and carbohydrate, thus water mobility was investigated using 1H-NMR spectroscopy. Electrolyte leakage indicated that tubers stored at 15°C for 1 year were partly injured and that frozen-thawed tissues were dead. Nuclear magnetic resonance (NMR) spin-lattice relaxation time (T1) and spin-spin relaxation time (T2) clearly increased with the duration of storage, whereas these values decreased in the dead tissues. Furthermore, Arrhenius plots for T1 and T2 were determined at temperatures ranging from 20 to 0°C in 2.5°C steps. In the fresh tubers, a strong converse temperature dependency was shown in the T2 measurement. On the contrary, there was no temperature dependency in the T2 of the dead tissues. Thus, the existence of inverse temperature dependency reflected tissue viability. Additionally, any change in the T2 of the fresh tubers occurred at about 14°C, which virtually coincided with the storage temperature of 15°C. The slope change in T2 might have responded to a physiological change as a primary event. In conclusion, monitoring water status by NMR could provide early identification of changes in the quality of post-harvest crops; this method shows great promise for use in environmental-stressed crop yield research.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalJournal of Agronomy and Crop Science
Volume190
Issue number1
DOIs
Publication statusPublished - Feb 1 2004

Fingerprint

sweet potatoes
nuclear magnetic resonance spectroscopy
tubers
temperature
water
methodology
storage temperature
electrolytes
crop yield
storage time
viability
carbohydrates
tissues
monitoring
crops

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

Cite this

1H-NMR Method Enables Early Identification of Degeneration in the Quality of Sweet Potato Tubers. / Iwaya-Inoue, M.; Matsui, R.; Sultana, N.; Saitou, K.; Sakaguchi, K.; Fukuyama, M.

In: Journal of Agronomy and Crop Science, Vol. 190, No. 1, 01.02.2004, p. 65-72.

Research output: Contribution to journalArticle

Iwaya-Inoue, M. ; Matsui, R. ; Sultana, N. ; Saitou, K. ; Sakaguchi, K. ; Fukuyama, M. / 1H-NMR Method Enables Early Identification of Degeneration in the Quality of Sweet Potato Tubers. In: Journal of Agronomy and Crop Science. 2004 ; Vol. 190, No. 1. pp. 65-72.
@article{d6decdc1601b47249e347547655af8c7,
title = "1H-NMR Method Enables Early Identification of Degeneration in the Quality of Sweet Potato Tubers",
abstract = "In sweet potato tuber, which is a tropical plant, long-term storage leads to loss of water and carbohydrate, thus water mobility was investigated using 1H-NMR spectroscopy. Electrolyte leakage indicated that tubers stored at 15°C for 1 year were partly injured and that frozen-thawed tissues were dead. Nuclear magnetic resonance (NMR) spin-lattice relaxation time (T1) and spin-spin relaxation time (T2) clearly increased with the duration of storage, whereas these values decreased in the dead tissues. Furthermore, Arrhenius plots for T1 and T2 were determined at temperatures ranging from 20 to 0°C in 2.5°C steps. In the fresh tubers, a strong converse temperature dependency was shown in the T2 measurement. On the contrary, there was no temperature dependency in the T2 of the dead tissues. Thus, the existence of inverse temperature dependency reflected tissue viability. Additionally, any change in the T2 of the fresh tubers occurred at about 14°C, which virtually coincided with the storage temperature of 15°C. The slope change in T2 might have responded to a physiological change as a primary event. In conclusion, monitoring water status by NMR could provide early identification of changes in the quality of post-harvest crops; this method shows great promise for use in environmental-stressed crop yield research.",
author = "M. Iwaya-Inoue and R. Matsui and N. Sultana and K. Saitou and K. Sakaguchi and M. Fukuyama",
year = "2004",
month = "2",
day = "1",
doi = "10.1046/j.1439-037X.2004.00076.x",
language = "English",
volume = "190",
pages = "65--72",
journal = "Journal of Agronomy and Crop Science",
issn = "0931-2250",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - 1H-NMR Method Enables Early Identification of Degeneration in the Quality of Sweet Potato Tubers

AU - Iwaya-Inoue, M.

AU - Matsui, R.

AU - Sultana, N.

AU - Saitou, K.

AU - Sakaguchi, K.

AU - Fukuyama, M.

PY - 2004/2/1

Y1 - 2004/2/1

N2 - In sweet potato tuber, which is a tropical plant, long-term storage leads to loss of water and carbohydrate, thus water mobility was investigated using 1H-NMR spectroscopy. Electrolyte leakage indicated that tubers stored at 15°C for 1 year were partly injured and that frozen-thawed tissues were dead. Nuclear magnetic resonance (NMR) spin-lattice relaxation time (T1) and spin-spin relaxation time (T2) clearly increased with the duration of storage, whereas these values decreased in the dead tissues. Furthermore, Arrhenius plots for T1 and T2 were determined at temperatures ranging from 20 to 0°C in 2.5°C steps. In the fresh tubers, a strong converse temperature dependency was shown in the T2 measurement. On the contrary, there was no temperature dependency in the T2 of the dead tissues. Thus, the existence of inverse temperature dependency reflected tissue viability. Additionally, any change in the T2 of the fresh tubers occurred at about 14°C, which virtually coincided with the storage temperature of 15°C. The slope change in T2 might have responded to a physiological change as a primary event. In conclusion, monitoring water status by NMR could provide early identification of changes in the quality of post-harvest crops; this method shows great promise for use in environmental-stressed crop yield research.

AB - In sweet potato tuber, which is a tropical plant, long-term storage leads to loss of water and carbohydrate, thus water mobility was investigated using 1H-NMR spectroscopy. Electrolyte leakage indicated that tubers stored at 15°C for 1 year were partly injured and that frozen-thawed tissues were dead. Nuclear magnetic resonance (NMR) spin-lattice relaxation time (T1) and spin-spin relaxation time (T2) clearly increased with the duration of storage, whereas these values decreased in the dead tissues. Furthermore, Arrhenius plots for T1 and T2 were determined at temperatures ranging from 20 to 0°C in 2.5°C steps. In the fresh tubers, a strong converse temperature dependency was shown in the T2 measurement. On the contrary, there was no temperature dependency in the T2 of the dead tissues. Thus, the existence of inverse temperature dependency reflected tissue viability. Additionally, any change in the T2 of the fresh tubers occurred at about 14°C, which virtually coincided with the storage temperature of 15°C. The slope change in T2 might have responded to a physiological change as a primary event. In conclusion, monitoring water status by NMR could provide early identification of changes in the quality of post-harvest crops; this method shows great promise for use in environmental-stressed crop yield research.

UR - http://www.scopus.com/inward/record.url?scp=1142293841&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1142293841&partnerID=8YFLogxK

U2 - 10.1046/j.1439-037X.2004.00076.x

DO - 10.1046/j.1439-037X.2004.00076.x

M3 - Article

AN - SCOPUS:1142293841

VL - 190

SP - 65

EP - 72

JO - Journal of Agronomy and Crop Science

JF - Journal of Agronomy and Crop Science

SN - 0931-2250

IS - 1

ER -