1H nuclear magnetic resonance study of the solution conformation of an antibacterial protein, sapecin

Hiroyuki Hanzawa, Ichio Shimada, Takashi Kuzuhara, Hiroto Komano, Daisuke Kohda, Fuyuhiko Inagaki, Shunji Natori, Yoji Arata

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The solution conformation of an antibacterial protein sapecin has been determined by 1H nuclear magnetic resonance (NMR) and dynamical simulated annealing calculations. It has been shown that the polypeptide fold consists of one flexible loop (residues 4-12), one helix (residues 15-23), and two extended strands (residues 24-31 and 34-40). It was found that the tertiary structure of sapecin is completely different from that of rabbit neutrophil defensin NP-5, which is homologous to sapecin in the amino acid sequences and also has the antibacterial activity. The three-dimensional structure determination has revealed that a basic-residue rich region and the hydrophobic surface face each other on the surface of sapecin.

Original languageEnglish
Pages (from-to)413-420
Number of pages8
JournalFEBS Letters
Volume269
Issue number2
DOIs
Publication statusPublished - Sep 3 1990

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Defensins
Protein Conformation
Conformations
Amino Acid Sequence
Neutrophils
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Rabbits
Peptides
Simulated annealing
Proteins
Amino Acids

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

1H nuclear magnetic resonance study of the solution conformation of an antibacterial protein, sapecin. / Hanzawa, Hiroyuki; Shimada, Ichio; Kuzuhara, Takashi; Komano, Hiroto; Kohda, Daisuke; Inagaki, Fuyuhiko; Natori, Shunji; Arata, Yoji.

In: FEBS Letters, Vol. 269, No. 2, 03.09.1990, p. 413-420.

Research output: Contribution to journalArticle

Hanzawa, H, Shimada, I, Kuzuhara, T, Komano, H, Kohda, D, Inagaki, F, Natori, S & Arata, Y 1990, '1H nuclear magnetic resonance study of the solution conformation of an antibacterial protein, sapecin', FEBS Letters, vol. 269, no. 2, pp. 413-420. https://doi.org/10.1016/0014-5793(90)81206-4
Hanzawa, Hiroyuki ; Shimada, Ichio ; Kuzuhara, Takashi ; Komano, Hiroto ; Kohda, Daisuke ; Inagaki, Fuyuhiko ; Natori, Shunji ; Arata, Yoji. / 1H nuclear magnetic resonance study of the solution conformation of an antibacterial protein, sapecin. In: FEBS Letters. 1990 ; Vol. 269, No. 2. pp. 413-420.
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